Journal ArticleDOI
Endogenous galectins and effect of galectin hapten inhibitors on the differentiation of the chick mesonephros
Kathryn M. Murphy,Sara E. Zalik +1 more
TLDR
Results suggest that galectin–glycoprotein interactions in the basement membrane are important in the maintenance of the renal epithelial phenotype.Abstract:
Galectins are galactoside-binding lectins. In the mesonephros of the chick embryo, the 16-kDa galectin is abundant in the glomerular and tubular basement membranes where it colocalizes with fibronectin and laminin. To test whether galectin–glycoprotein interactions could play a role in mesonephric development, the effects of the galectin hapten inhibitors thidodigalactoside (TDG) and lactose on the differentiation of the cultured mesonephros were investigated. When compared to control saccharide-free or maltose-treated cultures, mesonephroi cultured in the presence of TDG and lactose exhibited defects in tissue organization. These included a distorted tubule shape, pseudostratification of the tubular epithelium, and detachment of glomerular podocytes from the basement membrane. The presence of molecular differentiation markers in the developing mesonephros was investigated. In vivo, expression of the epithelial-specific cell adhesion molecule E-cadherin is restricted to differentiated tubular epithelial cells, whereas the intermediate filament protein vimentin is present in mesonephrogenic mesenchyme and is undetectable in tubular epithelial cells. In mesonephroi cultured in the absence of sugars or in the presence of maltose, the expression pattern of these two marker molecules resembles that found in the mesonephros in vivo. In contrast, in the mesonephroi cultured in the presence of TDG and lactose, the epithelial tubular cells expressing E-cadherin also express vimentin. Re-expression of vimentin in the tubular epithelial cells could indicate a partial reversal to a mesenchymal phenotype. Results suggest that galectin–glycoprotein interactions in the basement membrane are important in the maintenance of the renal epithelial phenotype. Dev Dyn 1999;215:248–263. © 1999 Wiley-Liss, Inc.read more
Citations
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Journal ArticleDOI
Galectin-3 modulates ureteric bud branching in organ culture of the developing mouse kidney
TL;DR: Evidence is provided that galectin-3 can modulate branching morphogenesis of the mouse ureteric bud/collecting duct lineage and the upregulation of galectIn-3 protein during kidney maturation, predominantly at sites where it could mediate cell/matrix interactions, seems to modulate growth of the ureTERic tree.
Journal ArticleDOI
Galectins in kidney development
TL;DR: This review will summarise recent progress in defining the roles of galectins that are expressed in the kidney in normal development, and discuss the evidence linking aberrant expression of galECTins with kidney disease.
Journal ArticleDOI
Characterization of the Xenopus galectin family. Three structurally different types as in mammals and regulated expression during embryogenesis.
TL;DR: The results show that the expression of each member is spatiotemporally regulated from eggs to adulthood, suggesting that galectins play multiple roles not only in adults, but also in development.
Journal ArticleDOI
Histology and ultrastructure of the equine lingual tonsil. I. Crypt epithelium and associated structures.
P. Kumar,J. F. Timoney +1 more
TL;DR: The microstructural and ultrastructural features of the equine lingual tonsil were studied in five young horses and showed strong affinity for Soybean, Phosphocarpus tetragonolobus 1, Ulex europaeus, and Griffonia simplicifolia 1 isolectin‐B4, which may be useful for embryological and microbiological investigations.
Journal ArticleDOI
Temporal and spatial regulation of expression of two galectins during kidney development of the chicken.
TL;DR: In this article, the presence of two avian galectins in liver (chicken galectin-16 CG-16) and intestine with different developmental regulation was ascertained using Western blot analysis.
References
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Journal ArticleDOI
A series of normal stages in the development of the chick embryo
TL;DR: The preparation of a series of normal stages of the chick embryo does not need justification at a time when chick ernbryos are not only widely used in descriptive and experimental embryology but are proving to be increasingly valuable in medical research, as in work on viruses and cancer.
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A series of normal stages in the development of the chick embryo
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A dot-immunobinding assay for monoclonal and other antibodies
TL;DR: A procedure for the assay of antibodies in sera based on the application of the antigen as a spot to nitrocellulose filters is described, which has the merit of being simpler in operation and more sensitive than comparable existing procedures.
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Galectins. Structure and function of a large family of animal lectins.
Journal ArticleDOI
Origins of Cell Polarity
David G. Drubin,W. James Nelson +1 more
TL;DR: The authors thank Kathryn Ayscough, Ken Beck, Fred Chang, Yih-Tai Chen, Kent Grindstaff, and Jeremy Thorner for comments on the manuscript as well as colleagues who kindly provided preprints.