Journal ArticleDOI
Enzymatic transesterification of soybean oil by using immobilized lipase on magnetic nano-particles
Wenlei Xie,Ning Ma +1 more
TLDR
In this article, the binding of lipase to magnetic particles was confirmed by enzyme assays, transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectra.Abstract:
Lipase was covalently immobilized onto magnetic Fe3O4 nano-particles by using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) as an activating agent, and the bound lipase was used to catalyze the transesterification of vegetable oils with methanol to produce fatty acid methyl esters. The binding of lipase to magnetic particles was confirmed by enzyme assays, transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectra. It was determined that the immobilized lipase exhibited better resistance to temperature and pH inactivation in comparison to free lipase. Using the immobilized lipase, the major parameters affecting the transesterification reaction, such as the alcohol/oil molar ratio, enzyme loading and free fatty acid present in reactants were investigated to obtain the optimum reaction condition. The conversion of soybean oil to methyl esters reached over 90% in the three-step transesterification when 40% immobilized lipase was used. Moreover, the lipase catalyst could be used for 3 times without significant decrease of the activity.read more
Citations
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Journal ArticleDOI
Conversion of microalgae to biofuel
Emma Suali,Rosalam Sarbatly +1 more
TL;DR: In this paper, the authors present an overall review of the use of microalgae as a biofuel feedstock and highlight the benefits of microalgal productivities under heterotrophic and phototrophic conditions.
Journal ArticleDOI
Lipase-catalyzed process for biodiesel production: Enzyme immobilization, process simulation and optimization
TL;DR: An overview of recent progresses in improving IL-catalyzed biodiesel production is provided, focusing on mid- and down-stream processing such as immobilization of lipase, bioreactors development, process optimization, simulation and techno-economic evaluation.
Journal ArticleDOI
Performance of calcium oxide as a heterogeneous catalyst in biodiesel production: A review
TL;DR: In this paper, various issues regarding CaO-catalyzed transesterification are reviewed, including the diverse performance of CaO in neat, loaded and mixed forms, as well as a support for other catalyst systems, CaO reaction mechanism, CAO tolerance to low to moderate oil qualities and reaction conditions, the conformance of CAO-calyzed biodiesel to key specifications and the future outlook and the challenges of the catalyst are suitably addressed.
Journal ArticleDOI
Application of magnetic nanoparticles in smart enzyme immobilization.
Hamideh Vaghari,Hoda Jafarizadeh-Malmiri,Mojgan Mohammadlou,Aydin Berenjian,Navideh Anarjan,Nahideh Jafari,Shahin Nasiri +6 more
TL;DR: This review provides an overview of recent developments in enzyme immobilization and stabilization protocols using magnetic nanocarriers and discusses the current applications and future growth prospects.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
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Fangrui Ma,Milford A. Hanna +1 more
TL;DR: In this article, the transesterification reaction is aected by molar ratio of glycerides to alcohol, catalysts, reaction temperature, reaction time and free fatty acids and water content of oils or fats.
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Advancements in development and characterization of biodiesel: A review
TL;DR: In this paper, the authors have discussed the development of biodiesel and its latest aspects of development, including biodegradability, kinetics involved in the process, and its stability have been critically reviewed.
Review article Advancements in development and characterization of biodiesel: A review
Y. C. Sharma,B. Singh +1 more
TL;DR: In this paper, the authors have discussed the development of biodiesel and its latest aspects of development, including biodegradability, kinetics involved in the process of the biodiesel production, and its stability.
Journal ArticleDOI
Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing
TL;DR: In this paper, a stepwise methanolysis system with immobilized Candida antarctica lipase was developed for the production of biodiesel fuel from waste oil, where the first-step reaction was conducted in the presence of 1/3 molar equivalent of MeOH for the stoichiometric amount.