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Escherichia coli is engineered to grow on CO2 and formic acid
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The engineered Escherichia coli strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO2 and formic acid alone.Abstract:
We engineered Escherichia coli to grow on CO2 and formic acid alone by introducing the synthetic CO2 and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo3 and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO2 and formic acid alone. Growth of Escherichia coli on carbon dioxide and formate is achieved by rational metabolic engineering alone.read more
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Synthetic biology 2020-2030: six commercially-available products that are changing our world
TL;DR: Six synthetic biology products that are now on the market are selected, highlighting the underlying technologies and projecting forward to the future that can be expected over the next ten years.
Journal ArticleDOI
Metabolic engineering strategies to enable microbial utilization of C1 feedstocks.
Wei Jiang,Wei Jiang,David Hernández Villamor,David Hernández Villamor,Huadong Peng,Jian Chen,Long Liu,Victoria S. Haritos,Rodrigo Ledesma-Amaro +8 more
TL;DR: One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing industry and have recently gained attention owing to their natural abundance, low production cost and availability as industrial by-products.
Journal ArticleDOI
Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
TL;DR: It is shown that engineering production of free fatty acids from sole methanol results in cell death with decreased cellular levels of phospholipids in the methylotrophic yeast Ogataea polymorpha, and cell growth is restored by adaptive laboratory evolution.
Journal ArticleDOI
Rescuing yeast from cell death enables overproduction of fatty acids from sole methanol
Journal ArticleDOI
Carbonic anhydrase for CO2 capture, conversion and utilization.
TL;DR: In this article , the authors discuss recent advances in carbonic anhydrase stabilization by protein engineering and enzyme immobilization, and shed light on state-of-the-art of in vitro and in vivo CA-mediated CO2 conversion for improved production of value-added chemicals using CO2 as a feedstock.
References
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TL;DR: An isothermal, single-reaction method for assembling multiple overlapping DNA molecules by the concerted action of a 5′ exonuclease, a DNA polymerase and a DNA ligase is described.
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The Electrochemical Reduction of Carbon Dioxide to Formate/Formic Acid: Engineering and Economic Feasibility
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Journal ArticleDOI
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Shmuel Gleizer,Roee Ben-Nissan,Yinon M. Bar-On,Niv Antonovsky,Elad Noor,Yehudit Zohar,Ghil Jona,Eyal Krieger,Melina Shamshoum,Arren Bar-Even,Ron Milo +10 more
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