Journal ArticleDOI
Establishment and characterization of a human acute monocytic leukemia cell line (THP‐1)
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TLDR
Results indicate that THP‐1 is a leukemic cell line with distinct monocytic markers, and the ability to restore T‐lymphocyte response to Con A.Abstract:
A human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia is described. This cell line had Fc and C3b receptors, but no surface or cytoplasmic immunoglobulins. HLA haplotypes of THP-1 were HLA-A2, -A9, -B5, -DRW1 and -DRW2. The monocytic nature of the cell line was characterized by: (1) the presence of alpha-naphthyl butyrate esterase activities which could be inhibited by NaF; (2) lysozyme production; (3) the phagocytosis of latex particles and sensitized sheep erythrocytes; and (4) the ability to restore T-lymphocyte response to Con A. The cells did not possess Epstein-Barr virus-associated nuclear antigen. These results indicate that THP-1 is a leukemia cell line with distinct monocytic markers. During culture, THP-1 maintained these monocytic characteristics for over 14 months.read more
Citations
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Journal ArticleDOI
High Resolution Mapping of the Binding Site on Human IgG1 for FcγRI, FcγRII, FcγRIII, and FcRn and Design of IgG1 Variants with Improved Binding to the FcγR
Robert L. Shields,Angela K. Namenuk,Kyu Hong,Y. Gloria Meng,Julie Rae,Josephine P. Briggs,Dong Xie,Jadine Lai,Andrew Stadlen,Betty Li,Judith A. Fox,Leonard G. Presta +11 more
TL;DR: Select IgG1 variants with improved binding to FcγRIIIA exhibited up to 100% enhancement in antibody-dependent cell cytotoxicity using human effector cells; these variants included changes at residues not found at the binding interface in the IgG/Fcγ RIIIA co-crystal structure.
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Nrf2 suppresses macrophage inflammatory response by blocking proinflammatory cytokine transcription
Eri H. Kobayashi,Takafumi Suzuki,Ryo Funayama,Takeshi Nagashima,Makiko Hayashi,Hiroki Sekine,Nobuyuki Tanaka,Takashi Moriguchi,Hozumi Motohashi,Keiko Nakayama,Masayuki Yamamoto +10 more
TL;DR: It is demonstrated that Nrf2 interferes with lipopolysaccharide-induced transcriptional upregulation of proinflammatory cytokines, including IL-6 and IL-1β, and establishes a molecular basis for an NRF2-mediated anti-inflammation approach.
Journal Article
Induction of Maturation in Cultured Human Monocytic Leukemia Cells by a Phorbol Diester
Shigeru Tsuchiya,Yasuko Kobayashi,Yoichi Goto,Hidesada Okumura,Shingi Nakae,Tasuke Konno,Keiya Tada +6 more
TL;DR: The results indicate that, after treatment with TPA, human monocytic leukemia cells may be converted into mature cells with functions of macrophages.
Journal ArticleDOI
The human leukemia cell line, THP-1: A multifacetted model for the study of monocyte-macrophage differentiation
TL;DR: Compared to other human myeloid cell lines, differentiated THP-1 cells behave more like native monocyte-derived macrophages, and provides a valuable model for studying the mechanisms involved in macrophage differentiation, and for exploring the regulation ofmacrophage-specific genes as they relate to physiological functions displayed by these cells.
Journal ArticleDOI
Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.
Charlotte M. McKee,Margaret B. Penno,Mary K. Cowman,Marie D. Burdick,Robert M. Strieter,Clare Bao,Paul W. Noble +6 more
TL;DR: The hypothesis that HA fragments generated during inflammation induce the expression of macrophage genes which are important in the development and maintenance of the inflammatory response is supported.
References
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Journal ArticleDOI
Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.
TL;DR: This CML cell-line represents a unique source of CML cells with meaningful indicators of malignancy for clinical and experimental studies.
Human chronic myelogenous leukemia cell-line with positive Philadelphia
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TL;DR: This CML cell-line represents a unique source of CML cells with meaningful indicators of malignancy for clinical and experimental studies.
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Establishment and characterization of a human histiocytic lymphoma cell line (U‐937)
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Journal ArticleDOI
Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
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Journal ArticleDOI
Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines.
Beverley M. Reedman,George Klein +1 more
TL;DR: The ACIF test was used as a tool to trace the Epstein‐Barr virus genome at the cellular level to study the complementfixing antigens of human lymphoblastoid cell lines.