Journal ArticleDOI
Evaluation of micro-CT for emphysema assessment in mice: comparison with non-radiological techniques
Xabier Artaechevarria,David Blanco,Gabriel de Biurrun,Mario Ceresa,Daniel Pérez-Martín,Gorka Bastarrika,Juan P. de Torres,Javier J. Zulueta,Luis M. Montuenga,Carlos Ortiz-de-Solorzano,Arrate Muñoz-Barrutia +10 more
TLDR
Histomorphometry is the most sensitive technique since it detects airspace enlargement before the other methods (1 h after treatment) and micro-CT correlates well with histology (r2 = 0.63) proving appropriate for longitudinal studies.Abstract:
Objectives
To define the potential, limitations and synergies of micro-CT and other non-radiological techniques for the quantification of emphysema and related processes in mice, by performing a complete characterization of the elastase-induced emphysema model.read more
Citations
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Journal ArticleDOI
In vivo small animal micro-CT using nanoparticle contrast agents.
TL;DR: This review focuses on new developments for in vivo small animal micro-CT using novel nanoparticle probes applied in preclinical research by adding spectral capabilities to separate multiple co-injected nanoparticles in vivo.
Journal ArticleDOI
Quantification of Lung Fibrosis and Emphysema in Mice Using Automated Micro-Computed Tomography
Ellen De Langhe,Greetje Vande Velde,Jeroen Hostens,Uwe Himmelreich,Benoit Nemery,Frank P. Luyten,Jeroen Vanoirbeek,Rik Lories +7 more
TL;DR: High resolution in vivo micro-computed tomography image analysis algorithm that runs fully automated and allows quantification of aerated lung volume in mice is shown to be a reliable quantitative tool to investigate experimental lung fibrosis and emphysema in mice.
Journal ArticleDOI
A Single Dose of Lipopolysaccharide into Mice with Emphysema Mimics Human Chronic Obstructive Pulmonary Disease Exacerbation as Assessed by Micro-Computed Tomography
Satoshi Kobayashi,Reiko Fujinawa,Fumi Ota,Shiho Kobayashi,Takashi Angata,Manabu Ueno,Toshitaka Maeno,Shinobu Kitazume,Keiichi Yoshida,Takeo Ishii,Congxiao Gao,Kazuaki Ohtsubo,Yoshiki Yamaguchi,Tomoko Betsuyaku,Kozui Kida,Naoyuki Taniguchi +15 more
TL;DR: A simple mouse model of COPD exacerbation that mimics human cases to some extent and will be useful for elucidating its mechanism and developing therapeutic strategies is used.
Journal ArticleDOI
Quantification of lung damage in an elastase-induced mouse model of emphysema
Arrate Muñoz-Barrutia,Mario Ceresa,Xabier Artaechevarria,Luis M. Montuenga,Carlos Ortiz-de-Solorzano +4 more
TL;DR: Micro-CT-derived descriptors are more sensitive than the other methods compared, to detect in vivo early signs of the disease.
Journal ArticleDOI
In vivo X-Ray Computed Tomographic Imaging of Soft Tissue with Native, Intravenous, or Oral Contrast
Connor A. Wathen,Nathan Foje,Tony Van Avermaete,Bernadette Miramontes,Sarah E. Chapaman,Todd A. Sasser,Raghuraman Kannan,Steven Gerstler,W. Matthew Leevy +8 more
TL;DR: The strategies available to visualize lung, fat, brain, kidney, liver, spleen, vasculature, gastrointestinal tract, and liver tissues of living mice are reviewed using either innate contrast, or commercial injectable or ingestible agents with selective perfusion.
References
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Cytokines in chronic obstructive pulmonary disease
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