Experimental Protection of Mice against Lethal Staphylococcus aureus Infection by Novel Bacteriophage ϕMR11
Shigenobu Matsuzaki,Masaharu Yasuda,Hiroshi Nishikawa,Masayuki Kuroda,Takako Ujihara,Taro Shuin,Yu-An Shen,Zhe Jin,Shigeyoshi Fujimoto,M. D Nasimuzzaman,Hiroshi Wakiguchi,Shigeyoshi Sugihara,Tetsuro Sugiura,Shigeki Koda,Asako Muraoka,Shosuke Imai +15 more
TLDR
The results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that phi MR11 may be a potential prototype for gene-modified, advanced therapeutic S.aureus phages.Abstract:
The protective effects of bacteriophages were assessed against experimental Staphylococcus aureus infection in mice. Of the S. aureus phages isolated in the study, phi MR11 was representatively used for all testing, because its host range was the most broad and it carries no genes for known toxins or antibiotic resistance. Intraperitoneal injections (8 x 10(8) cells) of S. aureus, including methicillin-resistant bacteria, caused bacteremia and eventual death in mice. In contrast, subsequent intraperitoneal administration of purified phi MR11 (MOI > or = 0.1) suppressed S. aureus-induced lethality. This lifesaving effect coincided with the rapid appearance of phi MR11 in the circulation, which remained at substantial levels until the bacteria were eradicated. Inoculation with high-dose phi MR11 alone produced no adverse effects attributable to the phage. These results uphold the efficacy of phage therapy against pernicious S. aureus infections in humans and suggest that phi MR11 may be a potential prototype for gene-modified, advanced therapeutic S. aureus phages.read more
Citations
More filters
Journal ArticleDOI
Methicillin-resistant Staphylococcus aureus: an evolutionary, epidemiologic, and therapeutic odyssey.
TL;DR: Methicillin-resistant Staphylococcus aureus has undergone rapid evolutionary changes and epidemiologic expansion and has led to an important change in the choice of antibiotics in the management of community-acquired infections and has also led to the development of novel antimicrobials.
Journal ArticleDOI
Evaluation of a Cocktail of Three Bacteriophages for Biocontrol of Escherichia coli O157:H7
TL;DR: It is expected that BIM formation should not hinder the use of these phages as biocontrol agents, particularly since low levels of the pathogen are typically encountered in the environment.
Journal ArticleDOI
Population and evolutionary dynamics of phage therapy.
Bruce R. Levin,James J. Bull +1 more
TL;DR: The population and evolutionary dynamics of bacterial–phage interactions that are relevant to phage therapy and prophylaxis are reviewed and illustrated with computer simulations.
Journal ArticleDOI
A historical overview of bacteriophage therapy as an alternative to antibiotics for the treatment of bacterial pathogens
TL;DR: The current therapeutic rationale and clinical experience with phage therapy as a treatment for invasive bacterial infection as novel alternative to antimicrobial chemotherapy is reviewed.
Journal ArticleDOI
Bacteriophage therapy : a revitalized therapy against bacterial infectious diseases
Shigenobu Matsuzaki,Mohammad Rashel,Jumpei Uchiyama,Shingo Sakurai,Takako Ujihara,Masayuki Kuroda,Masahiko Ikeuchi,Toshikazu Tani,Mikiya Fujieda,Hiroshi Wakiguchi,Shosuke Imai +10 more
TL;DR: Phage therapy will compensate for unavoidable complications of chemotherapy such as the appearance of multidrug resistance or substituted microbism, and phage-coded inhibitors of peptidoglycan synthesis (protein antibiotics), search methods for novel antibacterial agents using phage genome informatics, and vaccines utilizing phages or their products are being developed.
References
More filters
Journal ArticleDOI
Dissemination in Japanese hospitals of strains of Staphylococcus aureus heterogeneously resistant to vancomycin
Keiichi Hiramatsu,Nanae Aritaka,Hideaki Hanaki,Shiori Kawasaki,Yasuyuki Hosoda,Satoshi Hori,Yoshinosuke Fukuchi,Intetsu Kobayashi +7 more
TL;DR: Heterogeneously resistant VRSA was found in hospitals throughout Japan, which could explain, at least partly, the frequent therapeutic failure of MRSA infection with vancomycin in Japan.
Journal ArticleDOI
Bacteriophage lysis: mechanism and regulation.
TL;DR: The available evidence suggests that holins oligomerize to form nonspecific holes and that this hole-forming step is the regulated step in phage lysis, which is as much an essential feature of holin function as is the hole formation itself.
Journal ArticleDOI
A bacteriolytic agent that detects and kills Bacillus anthracis.
TL;DR: PlyG is shown that the PlyG lysin, isolated from the γ phage of B. anthracis, specifically kills B.Anthracis isolates and other members of the B. Anthracis ‘cluster’ of bacilli in vitro and in vivo.
Journal ArticleDOI
Prevention and elimination of upper respiratory colonization of mice by group A streptococci by using a bacteriophage lytic enzyme
TL;DR: It is shown that lysin represents a unique murein hydrolase that has a rapid lethal effect both in vitro and in vivo on group A streptococci, without affecting other indigenous microorganisms analyzed.
Journal ArticleDOI
Successful treatment of experimental Escherichia coli infections in mice using phage: its general superiority over antibiotics.
H W Smith,M B Huggins +1 more
TL;DR: A single intramuscular dose of one anti-K1 phage was more effective than multiple intamuscular does of tetracycline, ampicillin, chloramphenicol, or trimethoprim plus sulphafurazole in curing mice of a potentially lethal intramucularly or intracerebrally induced infection of MW; it was at least as effective as multiple intramUScular doses of streptomycin.
Related Papers (5)
Successful treatment of experimental Escherichia coli infections in mice using phage: its general superiority over antibiotics.
H W Smith,M B Huggins +1 more