Full recovery of the NADH:ubiquinone activity of complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica by the addition of phospholipids
TLDR
It is demonstrated that the inactivation of complex I from Y. lipolytica by general delipidation could be fully reversed simply by returning the phospholipids that had been removed during the purification procedure and no specific, functionally essential component had been lost.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 2002-10-03 and is currently open access. It has received 77 citations till now. The article focuses on the topics: Mitochondrial respiratory chain & Yarrowia.read more
Citations
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Journal ArticleDOI
Energy Converting NADH: Quinone Oxidoreductase (Complex I)
TL;DR: The coupling mechanism of complex I most likely involves semiquinone intermediates that drive proton pumping through redox-linked conformational changes.
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Decrease in Mitochondrial Complex I Activity in Ischemic/Reperfused Rat Heart. Involvement of Reactive Oxygen Species and Cardiolipin
Giuseppe Paradies,Giuseppe Petrosillo,Marilva Pistolese,Nicola Di Venosa,Antonio Federici,Francesca Maria Ruggiero +5 more
TL;DR: It is proposed that the defect in complex I activity in ischemic/reperfused rat heart could be ascribed to a ROS-induced cardiolipin damage, which may provide an explanation for some of the factors responsible for myocardial reperfusion injury.
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Functional role of cardiolipin in mitochondrial bioenergetics.
Giuseppe Paradies,Valeria Paradies,Valentina De Benedictis,Francesca Maria Ruggiero,Giuseppe Petrosillo +4 more
TL;DR: The current understanding of the functional role that cardiolipin plays in several reactions and processes involved in mitochondrial bioenergetics, including the respiratory chain complexes and substrate carrier proteins is discussed.
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Role of lipid peroxidation derived 4-hydroxynonenal (4-HNE) in cancer: focusing on mitochondria.
Huiqin Zhong,Huiyong Yin +1 more
TL;DR: Recent progress on the role of 4-HNE in pathogenesis of cancer is summarized and the involvement of mitochondria is focused on, with potential therapeutic strategies for targeting mitochondrial ROS generation, lipid peroxidation, and 4- HNE.
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Structure of Mammalian Respiratory Supercomplex I1III2IV1
TL;DR: The cryo-electron microscopy structure of the major SC in porcine heart suggests a possible mechanism by which electrons are transferred from NADH to cytochrome c and provides a platform for future functional dissection of respiration.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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MOLECULAR BASIS FOR MEMBRANE PHOSPHOLIPID DIVERSITY: Why Are There So Many Lipids?
TL;DR: The wide range of processes in which specific involvement of phospholipids has been documented explains the need for diversity inospholipid structure and why there are so many membrane lipids.
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Lipids of mitochondria
TL;DR: The lipid content and lipid composition of mitochondria, a comparison between mitochondrial and other cellular membranes, and mitocbondria from microorganisms are compared.
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Cardiolipins and biomembrane function
TL;DR: Evidence is discussed for roles of cardiolipins in oxidative phosphorylation mechanisms that regulate State 4 respiration by returning ejected protons across and over bacterial and mitochondrial membrane phospholipids, and that regulates State 3 respiration through the relative contributions of proteins that transport protons, electrons and/or metabolites.