Journal ArticleDOI
Functional Characterization of Human Nucleosome Assembly Protein-2 (NAP1L4) Suggests a Role as a Histone Chaperone
Pedro Rodriguez,David Munroe,Dirk Prawitt,Lee Lee Chu,Eva Bric,Jungho Kim,Laura H. Reid,Chris Davies,Hitoshi Nakagama,Ralf Loebbert,Andreas Winterpacht,Mary Jane Petruzzi,Michael J. Higgins,Norma J. Nowak,Glen A. Evans,Tom Shows,Bernard E. Weissman,Bernhard Zabel,David E. Housman,Jerry Pelletier +19 more
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TLDR
The results, coupled with tumor suppression assays in G401 WT cells, do not support a role for NAP-2 in the etiology of WT and indicate that it can shuttle between the cytoplasm and the nucleus, suggesting a role as a histone chaperone.About:
This article is published in Genomics.The article was published on 1997-09-15. It has received 92 citations till now. The article focuses on the topics: NAP1L4 & Nucleosome assembly.read more
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Identification of Novel MAP Kinase Pathway Signaling Targets by Functional Proteomics and Mass Spectrometry
Timothy S. Lewis,John B. Hunt,Lauren D. Aveline,Karen R. Jonscher,Donna F. Louie,Jennifer M. Yeh,Theresa Stines Nahreini,Katheryn A. Resing,Natalie G. Ahn,Natalie G. Ahn +9 more
TL;DR: This study combines functional proteomics with selective activation and inhibition of MKK1/2, in order to identify cellular targets regulated by the MKK/ERK cascade, and demonstrates the utility of this discovery-based strategy in elucidating novel MAP kinase pathway effectors.
Journal ArticleDOI
Histone chaperones: 30 years from isolation to elucidation of the mechanisms of nucleosome assembly and disassembly
TL;DR: An overview of prominent histone chaperones is provided with the goal of elucidating the mechanisms that preserve and modify epigenetic information and the reactions involved in nucleosome assembly and disassembly.
Journal ArticleDOI
The structure of nucleosome assembly protein 1.
Young-Jun Park,Karolin Luger +1 more
TL;DR: To the authors' knowledge, this is the first structure of a member of the large NAP family of proteins and suggests a mechanism by which the shuttling of histones to and from the nucleus is regulated.
Journal ArticleDOI
A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B
TL;DR: It is demonstrated that nucleosome assembly protein 1 (Nap1p), a protein previously implicated in the deposition of histones H2A and H2B, is also involved in the transport of these two histones, and a model in which Nap1p links the nuclear transport of H2 a and H 2B to chromatin assembly is proposed.
Journal ArticleDOI
Identification of Novel Imprinted Genes in a Genome-Wide Screen for Maternal Methylation
TL;DR: A genome-wide screen for novel imprinted loci is performed to identify differentially methylated regions (DMRs) methylated specifically on the maternal allele of parthenogenetic mouse embryos, and two of the three novel genes identified are located within the introns of other genes; their identification indicates that such "microimprinted" domains may be more common than previously thought.
References
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Journal ArticleDOI
DNA sequencing with chain-terminating inhibitors
TL;DR: A new method for determining nucleotide sequences in DNA is described, which makes use of the 2',3'-dideoxy and arabinon nucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase.
Journal ArticleDOI
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei
TL;DR: A procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters, including tRNA and Ad 2 VA, is developed.
Journal ArticleDOI
Nuclear targeting sequences--a consensus?
TL;DR: It is suggested in this review that, despite this diversity of nuclear targeting sequences, a consensus bipartite motif can be identified.
BookDOI
Chromatin structure and function
TL;DR: The Chromatin Pattern in Situ: Dependence upon Cell Cycle, Preimplantation and Development, and Cellular Aging in Vitro, and Generalized Biological Effects.
Journal ArticleDOI
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method
Jeffrey Field,J. Nikawa,Daniel Broek,B. MacDonald,Linda Rodgers,Ian A. Wilson,Richard A. Lerner,Michael Wigler +7 more
TL;DR: A method for immunoaffinity purification of Saccharomyces cerevisiae adenylyl cyclase based on creating a fusion with a small peptide epitope based on oligonucleotide technology is developed.
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