Showing papers in "Genomics in 1997"

TL;DR: FASTX and FASTY are used to scan the Mycoplasma genitalium, Haemophilus influenzae, and Methanococcus jannaschii genomes for unidentified or misidentified protein-coding genes and are found to be quite accurate, except when an out-of-frame translation produces a low-complexity protein sequence.

TL;DR: To characterize the regulation of NGAL further, cloned and sequenced a 5869-bp region of the NGAL gene including 1695 bp of the 5' nontranscribed region and a 3696-bp coding region encompassing seven exons and six introns.

TL;DR: The pattern of expression of GPCR-Br indicates that this receptor may be involved in physiologic responses specific to hormonally responsive tissues.

TL;DR: Northern blot analyses indicate that this gene is expressed at high levels in adult prostate and at a much lower level in testis, but is expressed little or not at all in several other tissues, and it is proposed that NKX3.1 is a candidate gene for playing a role in the opposing processes of androgen-driven differentiation of prostatic tissue and loss of that differentiation during the progression of prostate cancer.

TL;DR: A novel vascular endothelial growth factor (VEGF), VEGF-D, is identified and characterized, which is structurally related to vascular endothelium growth factor C and encode the eight cysteine residues that are highly conserved among the members of this family.

TL;DR: The deduced amino acid sequence of human myocilin showed significant homologies with nonmuscle myosin of Dictyostelium discoideum in the N- terminal region and also with olfactomedin of bullfrog in the C-terminal region, suggesting that myocILin is a novel cytoskeletal protein involved in the morphogenesis of ciliated neuroepithelium such as photoreceptor cells.

TL;DR: The recent cloning of a growth hormone secretagogue receptor from human pituitary gland and brain identified a third G protein-coupled receptor (GPC-R) involved in the control of growth hormone release.

TL;DR: The genomic organization of the HC gp-39 gene (HGMW-approved symbol CHI3L1) is characterized and its 5'-proximal promotor region is cloned and a variety of putative regulatory elements are identified.

TL;DR: Using selected trapped exons, a novel gene (named TMPRSS2) is identified that encodes a multimeric protein with a serine protease domain that is expressed strongly in small intestine and weakly in several other tissues.

TL;DR: A genetic linkage map of the canine genome has been developed by typing 150 microsatellite markers using 17 three-generation pedigrees, composed of 163 F2 individuals, to serve as a foundation for development of a comprehensive canine genetic map.

TL;DR: The authors' results provide very suggestive evidence for a susceptibility locus for schizophrenia in chromosome 6q from two independent data sets, and did not confirm, however, the previously described linkage in 6p, when tested in the systematic genome scanning data set.

TL;DR: Despite a complete lack of DNA sequence similarity and despite differences in their disulfide-pairing pattern, the α- and β-families appear to have evolved from a common premammalian defensin gene.

TL;DR: The AAT tool reduces the labor-intensive work of locating the exons of the query sequence and improves the process of defining intron-exon boundaries by using the wealth of available protein and cDNA data.

TL;DR: The AP-1 functional site is functional and responsible for the observed inducibility of the reporter gene by the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA), and no significative synergistic effect on CAT activity between theAP-1 and PEA-3 elements found in the collagenase-3 gene promoter was found.

TL;DR: The genomic organization of DSCR1 is determined and three additional alternative first exons are identified by RACE and cDNA library screening and Structural features of the conceptual protein encourage us to propose involvement of D SCR1 in the regulation of transcription and/or signal transduction.

TL;DR: The multipoint linkage analysis suggests that the novel ARVD locus, provisionally named ARVD4, maps to 2q32.3, within the chromosomal region including markers D 2S152, D2S103, and D2 S389.

TL;DR: The homozygous deletion of C438 in the coding sequence of ATP2A1 in Brody disease family 3, leading to a frameshift and truncation following Pro147 in SERCA1, is the fourth ATP2 a1 mutation to be associated with autosomal recessive Brody Disease.

TL;DR: The identification, cloning, and characterization of the human TBX1 gene is described, which maps to the center of the DiGeorge chromosomal region, and the mouse cDNA sequence is extended to permit comparisons between human and mouse Tbx1.

TL;DR: The isolation of a cDNA encoding the human homolog of Mdmx, which can bind p53 in vitro, and the ORF of the cDNA encodes a protein of 490 amino acids, 90% similar to mouse MDMx.

TL;DR: It is suggested that splice site suppression is responsible for the formation of two of the alternatively spliced forms of the mouse Lepr gene.

TL;DR: Two highly conserved human homologs of sina are characterized, suggesting their substantial evolutionary conservation, role in specifying cell fate, and activation in apoptotic cells suggest the SIAH proteins have important roles in vertebrate development.

TL;DR: It is shown that Nedd4 protein is localized to the cellular cytoplasm and maps to a previously defined homologous region between human and mouse chromosomes and thus provides additional information regarding interspecies comparative mapping.

TL;DR: A comprehensive analysis of protein domain families in Wormpep 11, which comprises 7299 proteins, finds that over two-thirds of the currently known human proteins are likely to have a homologue in the whole C. elegans genome and that a significant number of proteins are well conserved between C. Edwards and H. influenzae.

TL;DR: Northern blot analysis demonstrated that RAD51B mRNA is widely expressed and most abundant in tissues active in recombination, and functions associated with known RAD51 homologs suggest a role for RAD 51B in meiotic recombination and/or recombinational repair.

TL;DR: Full-length Coch5B2 deduced amino acid sequences reveal a very high degree of conservation in the coding region, with message detected in mouse adult spleen, cerebrum, cerebellum/medulla, and thymus and in both species very low level expression is detected in total eye.

TL;DR: Two new vertebrate calpain genes are identified, Capn5 and Capn6, which highlight a hitherto unknown complexity of the calpain family with subclasses perhaps possessing different modes of regulation.

TL;DR: Analysis of the 5' upstream sequence and the structure of the PSEN1 gene reveals that the5' sequence contains multiple putative transcription regulatory elements including clusters of STAT elements involved in transcriptional activation in response to signal transduction.

TL;DR: The transcription of MAGES-B1 and MAGE-B2 can be induced by 5-aza-2'-deoxycytidine, suggesting that the activation of these genes in tumors results from a demethylation process.

TL;DR: The fact that all currently described genes encoding defensins map to chromosome 8p21-pter suggests that a gene cluster in this chromosomal region may play a major role in antimicrobial defense.

TL;DR: It is shown that Nkrp1 genes are clustered in a region flanked by A2m and Cd69 genes and that most Ly49 genes are clusters in a distal region -1 Mb distant, and syntenic intervals of mouse chromosome 6 and human chromosome 12p that include the NKC are conserved.