Journal ArticleDOI
Functional expression of five Arabidopsis fatty acyl-CoA reductase genes in Escherichia coli
Thuy T.P. Doan,Anders S. Carlsson,Mats Hamberg,Leif Bülow,Sten Stymne,Peter Olsson,Peter Olsson +6 more
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TLDR
The five functional FAR enzymes yielded distinctly different compositions of fatty alcohols when expressed in E. coli, indicating that the different enzymes may be involved in the production of different types of alcohols in plant cells.About:
This article is published in Journal of Plant Physiology.The article was published on 2009-05-15. It has received 159 citations till now. The article focuses on the topics: Arabidopsis & Fatty alcohol.read more
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Microbial Biosynthesis of Alkanes
TL;DR: The discovery of an alkane biosynthesis pathway in cyanobacteria that converts intermediates of fatty acid metabolism to alkanes and alkenes is described and is likely to be a valuable tool in the production of biofuels.
Journal ArticleDOI
Genetic Regulation of Sporopollenin Synthesis and Pollen Exine Development
Tohru Ariizumi,Kinya Toriyama +1 more
TL;DR: The currently accepted understanding of the molecular regulation of sporopollenin biosynthesis is reviewed and unanswered questions regarding the requirements underpinning proper exine pattern formation are examined, as based on genetic evidence.
Journal ArticleDOI
Metabolic engineering of Saccharomyces cerevisiae for production of fatty acid-derived biofuels and chemicals.
TL;DR: This work engineered the budding yeast Saccharomyces cerevisiae to produce fatty acid-derived biofuels and chemicals from simple sugars and envision that this approach will provide a scalable, controllable and economic route to this important class of chemicals.
Journal ArticleDOI
New microbial fuels: a biotech perspective.
Mathew Rude,Andreas W. Schirmer +1 more
TL;DR: Yields and efficiencies of the four metabolic pathways leading to these microbial fuels-mostly designed and optimized in Escherichia coli and Saccharomyces cerevisiae using modern tools of metabolic engineering and synthetic biology-and the robustness of the biocatalysts that convert the metabolic intermediates to finished and engine-ready fuels will determine if they can be commercially successful and contribute to alleviating the authors' dependence on fossil fuels.
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CYP704B1 Is a Long-Chain Fatty Acid ω-Hydroxylase Essential for Sporopollenin Synthesis in Pollen of Arabidopsis
Anna A. Dobritsa,Jay Shrestha,Marc Morant,Franck Pinot,Michiyo Matsuno,Robert Swanson,Birger Lindberg Møller,Daphne Preuss +7 more
TL;DR: It is shown that different classes of hydroxylated fatty acids serve as essential compounds for sporopollenin formation, and the genetic relationships between CYP704B1, CYP703A2, and another exine gene, MALE STERILITY2, which encodes a fatty acyl reductase, were explored.
References
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Gapped BLAST and PSI-BLAST: a new generation of protein database search programs.
Stephen F. Altschul,Thomas L. Madden,Alejandro A. Schäffer,Jinghui Zhang,Zheng Zhang,Webb Miller,David J. Lipman +6 more
TL;DR: A new criterion for triggering the extension of word hits, combined with a new heuristic for generating gapped alignments, yields a gapped BLAST program that runs at approximately three times the speed of the original.
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
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Base-calling of automated sequencer traces using Phred. I. accuracy assessment
TL;DR: In this article, a base-calling program for automated sequencer traces, phred, with improved accuracy was proposed. But it was not shown to achieve a lower error rate than the ABI software, averaging 40%-50% fewer errors in the data sets examined independent of position in read, machine running conditions, or sequencing chemistry.