Journal ArticleDOI
Gas liquid radiochromatography of intact natural triglycerides.
W. C. Breckenridge,Arnis Kuksis +1 more
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Gas liquid radiochromatography was successfully applied to the simultaneous analysis of mass and radioactivity of intact standard and natural triglycerides labeled with14C in their fatty acid or glycerol moieties and showed that the detected radioactivity was proportional to the mass and not the moles of the labeled triglyceride measured in the hydrogen flame ionization detector.Abstract:
Gas liquid radiochromatography was successfully applied to the simultaneous analysis of mass and radioactivity of intact standard and natural triglycerides labeled with14C in their fatty acid or glycerol moieties. Quantitative measurements of mass and radioactivity were made with a gas chromatograph equipped with dual glass columns, stream splitters, combustion train, and a proportional gas counter. Under optimum conditions, as little as 500 cpm/peak of tristearin could be detected with a relative error of 10%. At higher counting rates the error was less than 5% and the achieved resolution of radioactivity was only slightly inferior to that of the mass. The counting efficiency of the system was better than 90%. The study showed that the detected radioactivity was proportional to the mass and not the moles of the labeled triglyceride measured in the hydrogen flame ionization detector. Practical application of the system is limited by the need for samples of relatively high specific activity (500–1000 dpm/50 μg/peak).read more
Citations
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Journal ArticleDOI
Regulation of fatty acid synthesis in isolated hepatocytes prepared from the livers of neonatal chicks.
TL;DR: The hypothesis that fatty acid synthesis is regulated by the intracellular concentration of a metabolite derived from free fatty acids, probably long chain fatty acyl-CoA, is supported.
Journal ArticleDOI
On the Relationship between Fatty Acid Synthesis and the Total Activities of Acetyl Coenzyme A Carboxylase and Fatty Acid Synthetase in the Liver of Prenatal and Early Postnatal Chicks
TL;DR: The results suggest that acetyl-CoA carboxylase and fatty acid synthetase are not part of an "operon" or its eukaryote equivalent, and that changes in their total activities did not initiate changes in the rate of fatty acid synthesis.
Journal ArticleDOI
Quantitative capillary gas—liquid chromatography of triglycerides on a fused-silica column with a chemically bonded stationary phase
P. Maresˇ,P. Husˇek +1 more
TL;DR: In this paper, the effect of sample injection technique, carrier gas for-rate, amount and molecular weight of substance and temperature progamming on the values of the weight correction factor, ƒ w, were studied for model samples consisting of identical amounts of saturated triglycerides with carbon numbers of 30, 36, 42, 48, 54 and 60.
Journal ArticleDOI
Differences in metabolism of cholesterol and sitosterol following intravenous injection in rats.
M.T.R. Subbiah,Arnis Kuksis +1 more
TL;DR: There were differences in the fatty acid composition of the steryl esters, in the extent of ester formation, as well as in the proportion and nature of bile acids derived from the two sterols.
References
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Journal ArticleDOI
A rapid method of total lipid extraction and purification.
E. G. Bligh,W. J. Dyer +1 more
TL;DR: The lipid decomposition studies in frozen fish have led to the development of a simple and rapid method for the extraction and purification of lipids from biological materials that has been applied to fish muscle and may easily be adapted to use with other tissues.
Journal ArticleDOI
Tumor lipids: Metabolic relationships derived from structural analyses of acyl, alkyl, and alk-1-enyl moieties of neutral glycerides and phosphoglycerides☆
Randall Wood,Fred Snyder +1 more
TL;DR: The similarities in composition at both the 1- and 2-positions between triglycerides and diacyl PC and between GEDE and alkyl acyl PC suggest a loss of acyl CoA: lysophosphatide acyl trausferase enzymes which are present in normal tissue.