Genetic inactivation of European sea bass (Dicentrarchus labrax L.) eggs using UV-irradiation: observations and perspectives.
Julie Colléter,David J. Penman,Stephane Lallement,Christian Fauvel,Tanja Lexau Hanebrekke,Renate Døving Osvik,Hans Christian Eilertsen,Helena D'Cotta,Béatrice Chatain,Stefano Peruzzi +9 more
TLDR
The conditions for the genetic inactivation of the maternal genome in the European sea bass were explored using different combinations of UV irradiation levels and durations and revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids with known UV-screening properties.Abstract:
Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ x cm(-2)), only one dose (60 mJ x cm(-2) x min(-1) with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.read more
Citations
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Journal ArticleDOI
The European sea bass: a key marine fish model in the wild and in aquaculture.
TL;DR: The domestication process must be better understood to improve production and also to fully evaluate the possible impact of aquaculture escapees on wild populations, the latter of which is an important question for all large‐scale Aquaculture productions.
Journal ArticleDOI
De novo synthesis of a sunscreen compound in vertebrates
Andrew R. Osborn,Khaled H. Almabruk,Garrett Holzwarth,Shumpei Asamizu,Jane LaDu,Kelsey Kean,P. Andrew Karplus,Robert L. Tanguay,Alan T. Bakalinsky,Taifo Mahmud +9 more
TL;DR: The unexpected discovery that fish can synthesize gadusol de novo is reported and that the analogous pathways are also present in amphibians, reptiles, and birds and it is demonstrated that engineered yeast containing the fish genes can produce and secrete gadusols.
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A comparative analysis of bipolar and freehand experimental knapping products from Olduvai Gorge, Tanzania
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