Journal ArticleDOI
Genome engineering for crop improvement and future agriculture
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TLDR
This article reviewed the development and application of genome editing tools in plants while highlighting newly developed techniques and discussed their impact on crop production, with an emphasis on recent advancements in genome editing-based plant improvements that could not be achieved by conventional breeding.About:
This article is published in Cell.The article was published on 2021-03-18. It has received 272 citations till now. The article focuses on the topics: Genome editing & Population.read more
Citations
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Journal ArticleDOI
Precise plant genome editing using base editors and prime editors.
TL;DR: A review of the current status of base editors and prime editors in plants is provided in this paper, which summarizes both technological developments and biological applications of these precise genome editors, including base editing and prime editing technologies.
Journal ArticleDOI
Genome-edited powdery mildew resistance in wheat without growth penalties
Shengnan Li,Dexing Lin,Yunwei Zhang,Min Deng,Yongxing Chen,Bin Lv,Boshu Li,Yuan Lei,Yanpeng Wang,Long Zhao,Yueting Liang,Jinxing Liu,Kunling Chen,Zhiyong Liu,Jun Xiao,Jin-Long Qiu,Caixia Gao +16 more
Journal ArticleDOI
The CRISPR-Cas toolbox and gene editing technologies
TL;DR: A comprehensive review of CRISPR-Cas-related technologies can be found in this article , where the authors introduce and summarize the characteristics and applications of different types of CRispr-Cas tools.
Journal ArticleDOI
Genome-wide specificity of prime editors in plants.
Shuai Jin,Qiupeng Lin,Yingfeng Luo,Zixu Zhu,Guanwen Liu,Yunjia Li,Kunling Chen,Jin-Long Qiu,Caixia Gao +8 more
TL;DR: In this paper, the mismatch tolerance of PEs in plant cells was examined and the editing frequency was influenced by the number and location of mismatches in the primer binding site and spacer of the prime editing guide RNA (pegRNA).
Journal ArticleDOI
Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture.
Tingdong Li,Hu Jiacheng,Yu Sun,Boshu Li,Dingliang Zhang,Wenli Li,Jinxing Liu,Dawei Li,Caixia Gao,Yongliang Zhang,Yanpeng Wang +10 more
TL;DR: In this paper, a Barley stripe mosaic virus-based sgRNA delivery vector (BSMV-sg) was used to perform heritable genome editing in Cas9-transgenic wheat plants.
References
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Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Alexis C. Komor,Yongjoo Kim,Yongjoo Kim,Michael S. Packer,Michael S. Packer,John A. Zuris,John A. Zuris,David R. Liu,David R. Liu +8 more
TL;DR: E engineered fusions of CRISPR/Cas9 and a cytidine deaminase enzyme that retain the ability to be programmed with a guide RNA, do not induce dsDNA breaks, and mediate the direct conversion of cytidine to uridine, thereby effecting a C→T (or G→A) substitution.
Journal ArticleDOI
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
Nicole M. Gaudelli,Alexis C. Komor,Holly A. Rees,Holly A. Rees,Holly A. Rees,Michael S. Packer,Ahmed H. Badran,Ahmed H. Badran,Ahmed H. Badran,David I. Bryson,David R. Liu,David R. Liu,David R. Liu +12 more
TL;DR: Adenine base editors (ABEs) that mediate the conversion of A•T to G•C in genomic DNA are described and a transfer RNA adenosine deaminase is evolved to operate on DNA when fused to a catalytically impaired CRISPR–Cas9 mutant.
Journal ArticleDOI
Search-and-replace genome editing without double-strand breaks or donor DNA
Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Jessie Rose Davis,Jessie Rose Davis,Jessie Rose Davis,Alexander A. Sousa,Alexander A. Sousa,Alexander A. Sousa,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Jonathan M. Levy,Jonathan M. Levy,Jonathan M. Levy,Peter J. Chen,Peter J. Chen,Peter J. Chen,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Aditya Raguram,Aditya Raguram,Aditya Raguram,David R. Liu,David R. Liu,David R. Liu +32 more
TL;DR: A new DNA-editing technique called prime editing offers improved versatility and efficiency with reduced byproducts compared with existing techniques, and shows potential for correcting disease-associated mutations.
Journal ArticleDOI
Targeted genome modification of crop plants using a CRISPR-Cas system.
Qiwei Shan,Yanpeng Wang,Jun Li,Yi Zhang,Kunling Chen,Zhen Liang,Kang Zhang,Jinxing Liu,Jianzhong Jeff Xi,Jin-Long Qiu,Caixia Gao +10 more
TL;DR: This work, together with the mice work, demonstrates that it should be feasible to produce genetargeted models in rodents and probably other mammalian species using the CRISPRCas systems.
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