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Green (cell) factories for advanced production of plant secondary metabolites

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TLDR
An integrated overview of the possible avenues for enhancing the biosynthesis of high-value marketable molecules produced by plant in vitro systems are presented, including metabolic engineering and CRISPR/Cas9 technology for the regulation of plant metabolism through overexpression/repression of single or multiple structural genes or transcriptional factors.
Abstract
For centuries plants have been intensively utilized as reliable sources of food, flavoring, agrochemical and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due t...

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Green Silver and Gold Nanoparticles: Biological Synthesis Approaches and Potentials for Biomedical Applications.

TL;DR: In this paper, a comprehensive collection of the most recent green methodologies, surveys the major nanoparticle characterization techniques and screens the effects triggered by the obtained nanomaterials in various living systems to give an impression on the biomedical potential of green synthesized silver and gold nanoparticles.
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Rosmarinic acid - From bench to valuable applications in food industry

TL;DR: The present review will explore the contemporary biotechnological approaches for RA mass-production and will attempt to summarize its main biological properties based on recent studies, suggesting its value as food additive should be thoroughly evaluated.
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Anthocyanins, multi-functional natural products of industrial relevance: Recent biotechnological advances

TL;DR: Various challenges and ways of investigation are proposed here to improve anthocyanin production by using the in vitro plant cell or tissue culture and metabolic engineering of plants and microbial culture systems, which can be further utilized for pharmaceutical, cosmetics and food applications.
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Production of bioactive plant secondary metabolites through in vitro technologies—status and outlook

TL;DR: A review of recent advances in the in vitro production of high-value plant secondary metabolites of medicinal importance can be found in this article, where the authors summarize the most important advances in this area.
References
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Journal ArticleDOI

Natural Products as Sources of New Drugs from 1981 to 2014

TL;DR: This contribution is a completely updated and expanded version of the four prior analogous reviews that were published in this journal in 1997, 2003, 2007, and 2012, and the time frame has been extended to cover the 34 years from January 1, 1981, to December 31, 2014, for all diseases worldwide, and from 1950 (earliest so far identified) to December 2014 for all approved antitumor drugs worldwide.
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NMR-based metabolomic analysis of plants.

TL;DR: This protocol is particularly suited for the analysis of secondary metabolites such as phenolic compounds and for primary metabolites, and is rapid; it takes not more than 30 min for sample preparation and a further 10 min for NMR spectrum acquisition.
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Current approaches and challenges for the metabolite profiling of complex natural extracts

TL;DR: A survey of the techniques that are used for generic and comprehensive profiling of secondary metabolites in natural extracts is provided and the structural information that can be generated through these techniques or in combination is compared in relation to the identification of metabolites in complex mixtures.
Journal ArticleDOI

Verbascoside — A review of its occurrence, (bio)synthesis and pharmacological significance

TL;DR: Verbascoside is a phenylethanoid glycoside that was first isolated from mullein but is also found in several other plant species as mentioned in this paper, including genetically transformed roots.
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