Journal ArticleDOI
Growth promotive conditions for enhanced eritadenine production during submerged cultivation of Lentinus edodes
TLDR
In this article, a water extract of distillers dried grains with solubles (DDGS) was added to the culture media to increase the mycelial biomass and in turn the production of eritadenine.Abstract:
BACKGROUND: Mycelium of the medicinal mushroom shiitake, Lentinus edodes, is a potential source for production of the blood cholesterol reducing compound eritadenine. To increase the mycelial biomass and in turn the production of eritadenine, a potential growth promoting substance in the form of a water extract of distillers dried grains with solubles (DDGS) was added to the culture media.
RESULTS: The hot water extract of DDGS was shown to considerably increase the growth of shiitake mycelia in bioreactor cultivations; the mycelial yield was 2–3 times higher than in the control, and the highest final biomass concentration obtained was 3.4 g L−1. Further, by using shake flask cultures as inoculums the bioreactor cultivation time could be reduced by 1 week for some of the experiments. The highest final titer of eritadenine in the present study was 25.1 mg L−1, which was about 2 times higher than in the control, and was also obtained when a water extract of DDGS was added to the culture medium.
CONCLUSION: It was demonstrated that a water extract of DDGS promoted the growth of shiitake mycelia in bioreactor cultivations, along with enhanced eritadenine production. Copyright © 2012 Society of Chemical Industryread more
Citations
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Journal ArticleDOI
Bioactive metabolites from macrofungi: ethnopharmacology, biological activities and chemistry
Dilani D. De Silva,Sylvie Rapior,Enge Sudarman,Marc Stadler,Jianchu Xu,S. Aisyah Alias,Kevin D. Hyde,Kevin D. Hyde +7 more
TL;DR: This review compile the information on bioactive structure-elucidated metabolites from macrofungi discovered over the last decade and highlight their unique chemical diversity and potential benefits to novel drug discovery.
Journal ArticleDOI
Medicinal importance of mushroom mycelium: Mechanisms and applications
TL;DR: This review focuses on the optimum growth conditions required by mycelia for enhanced quality and yield prospects along with the biological mechanisms responsible for their therapeutic properties and their applications.
Journal ArticleDOI
Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)
Byron Durán-Rivera,José Rodrigo Moreno-Suárez,Felipe Rojas Rodas,Kelly Marcela Valencia Jiménez,Dagoberto Castro–Restrepo +4 more
TL;DR: In this paper, the effect of three carbon sources (mannitol, minced potato and sucrose), two immobilization substrates (alginate and wood cylinders), and three surfactants (Tween 20, Tween 40 and Tween 80), were evaluated on eritadenine production using shiitake (Lentinula edodes) mycelium under submerged cultivation, in shake flasks within 20 days.
Book ChapterDOI
Advances in Production of Medicinal Mushrooms Biomass in Solid State and Submerged Bioreactors.
Marin Berovič,Jian-Jiang Zhong +1 more
Journal ArticleDOI
Molecular identification of Shiitake [Lentinula edodes Berk (Pegler)] and production of secondary metabolites with biotechnological potential
Byron Duran-Rivera,Felipe Rojas-Rodas,Wilber Silva-López,Crhistian Gómez-Suárez,Dagoberto Castro-Restrepo +4 more
TL;DR: Low genetic diversity in the four shiitake isolates were identified, corresponding to two haplotypes with minimal genetic difference between them, related to the Japanese strain Cr62, indicating that Colombian farmers cultivate almost the same strains of shitake.
References
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Journal ArticleDOI
Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage.
Youngmi Kim,Nathan S. Mosier,Rick Hendrickson,Thaddeus Chukwuemeka Ezeji,Hans P. Blaschek,Bruce S. Dien,Michael A. Cotta,Bruce E. Dale,Michael R. Ladisch +8 more
TL;DR: Polymeric sugars, crude protein, crude oil and ash contents of DDGS and wet distillers' grains were accurately and reproducibly determined by the compositional analysis procedure described in this paper.
Journal ArticleDOI
Lentinus edodes and Pleurotus species lignocellulolytic enzymes activity in submerged and solid-state fermentation of lignocellulosic wastes of different composition.
Vladimir Elisashvili,Michel Penninckx,Eva Kachlishvili,Nino Tsiklauri,Eka Metreveli,Tamar Kharziani,G. Kvesitadze +6 more
TL;DR: The study pointed out that the nature of lignocellulosic material and the method of fungi cultivation are factors determining the expression of lignecellulolytic potential of fungi as well as the ratio of individual enzymes in enzyme complex.
Journal ArticleDOI
Effect of initial pH on production of ganoderic acid and polysaccharide by submerged fermentation of Ganoderma lucidum
Qinghua Fang,Jian-Jiang Zhong +1 more
TL;DR: The effects of initial pH on simultaneous production of ganoderic acid and a polysaccharide by the fungus Ganoderma lucidum were investigated in shake flasks and lowering the initial pH from 6.5 to 3.5 gradually led to a higher production of extracellular poly Saccharide and a higher specific production of intracellular poly saccharide.
Journal ArticleDOI
Pellet morphology, culture rheology and lovastatin production in cultures of Aspergillus terreus.
J.L. Casas López,J.A. Sánchez Pérez,J. M. Fernández Sevilla,E.M. Rodríguez Porcel,Yusuf Chisti +4 more
TL;DR: Both an upper limit on agitation intensity and a high level of dissolved oxygen are essential for attaining high titers of lovastatin in fungal pellet morphology and broth rheology.
Journal ArticleDOI
Role of oxygen supply in submerged fermentation of Ganoderma lucidum for production of Ganoderma polysaccharide and ganoderic acid
Ya-Jie Tang,Jian-Jiang Zhong +1 more
TL;DR: The cell growth of G. lucidum was significantly inhibited when DOT was controlled ∼10% of air saturation, which was due to the oxygen limitation in mycelia aggregates, and the total production and productivity of IPS and GA at a low DOT were lower than those at a high DOT.