Guidelines for the validation and application of typing methods for use in bacterial epidemiology
A. van Belkum,Panayotis T. Tassios,Lenie Dijkshoorn,Sara Hæggman,Barry Cookson,Norman K. Fry,V. Fussing,Jonathan Green,Edward J. Feil,Peter Gerner-Smidt,Sylvain Brisse,Marc Struelens +11 more
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TLDR
Newer and older, phenotypic and genotypic methods for typing of all clinically relevant bacterial species are described according to their principles, advantages and disadvantages and Criteria for their evaluation and application and the interpretation of their results are proposed.About:
This article is published in Clinical Microbiology and Infection.The article was published on 2007-01-01 and is currently open access. It has received 771 citations till now.read more
Citations
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Journal ArticleDOI
Multiresistant Gram-negative bacteria: the role of high-risk clones in the dissemination of antibiotic resistance
TL;DR: 'high-risk clones' play a major role in the spread of resistance, with the risk lying in their tenacity--deriving from poorly understood survival traits--and a flexible ability to accumulate and switch resistance, rather than to constant resistance batteries.
Harmonization of pulsed-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its applicatio
Murchan S,Kaufmann M E,Deplano A,Ryck de R,Struelens M,Zinn C E,Fussig,Salmenlinna S,Vuopio-Varkila J,El Solh N,Cuny C,Witte W,Tassios P T,Legakis N,Leeuwen van W,Belkum van A,Vindel A,Laconcha I,Garaizar J,Haeggman S,Olsson-Liljequist B,Ransjo U,Coombes G,Cookson B +23 more
TL;DR: In this article, the authors compared different European pulsed-fieldgel electrophoresis (PFGE) protocols for MRSA and of the various national MRSA clones themselves.
Journal ArticleDOI
Overview of molecular typing methods for outbreak detection and epidemiological surveillance
Artur J. Sabat,Ana Budimir,Nashev D,Raquel Sá-Leão,van Dijl Jm,Frédéric Laurent,Hajo Grundmann,Alexander W. Friedrich +7 more
TL;DR: Current and new molecular typing methods for outbreak detection and epidemiological surveillance of bacterial pathogens in clinical practice are reviewed to give an overview of their specific advantages and disadvantages.
Journal ArticleDOI
Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes
Alexandra Moura,Alexis Criscuolo,Hannes Pouseele,Mylène M. Maury,Alexandre Leclercq,Alexandre Leclercq,Cheryl L. Tarr,Jonas T. Björkman,Timothy J. Dallman,Aleisha Reimer,Vincent Enouf,Elise Larsonneur,Heather A. Carleton,Hélène Bracq-Dieye,Hélène Bracq-Dieye,Lee S. Katz,Louis Jones,Marie Touchon,Mathieu Tourdjman,Matthew G. Walker,Steven Stroika,Thomas Cantinelli,Viviane Chenal-Francisque,Zuzana Kucerova,Eduardo P. C. Rocha,Celine Nadon,Kathie Grant,Eva Møller Nielsen,Bruno Pot,Peter Gerner-Smidt,Marc Lecuit,Sylvain Brisse +31 more
TL;DR: This work demonstrates the urgent need to monitor Lm strains at the global level and provides the unified approach needed for global harmonization of Lm genome-based typing and population biology.
Journal ArticleDOI
Ribosomal multilocus sequence typing: universal characterization of bacteria from domain to strain.
Keith A. Jolley,Carly M. Bliss,Julia S. Bennett,Holly B. Bratcher,Carina Brehony,Frances M. Colles,Helen M. L. Wimalarathna,Odile B. Harrison,Samuel K. Sheppard,Alison J. Cody,Martin C. J. Maiden +10 more
TL;DR: This work proposes ribosomal multilocus sequence typing (rMLST), an approach which indexes variation of the 53 genes encoding the bacterial ribosome protein subunits (rps genes), as a means of integrating microbial genealogy and typing, and employs curated reference sequences to identify gene variants efficiently and rapidly.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
AFLP: a new technique for DNA fingerprinting.
Pieter Vos,René Cornelis Josephus Hogers,Marjo Bleeker,Martin Reijans,Theo van de Lee,Miranda Hornes,Adrie Friters,Jerina Pot,Johan Paleman,Martin Kuiper,Marc Zabeau +10 more
TL;DR: The AFLP technique provides a novel and very powerful DNA fingerprinting technique for DNAs of any origin or complexity that allows the specific co-amplification of high numbers of restriction fragments.
Journal ArticleDOI
Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
Fred C. Tenover,Robert D. Arbeit,Richard V. Goering,P. A. Mickelsen,Barbara E Murray,D. H. Persing,B. Swaminathan +6 more
TL;DR: This research presents a novel, scalable and scalable approach that allows for real-time assessment of the severity of the infection and its impact on patients’ health.
Journal ArticleDOI
Multilocus sequence typing: A portable approach to the identification of clones within populations of pathogenic microorganisms
Martin C. J. Maiden,Jane A. Bygraves,Edward J. Feil,Giovanna Morelli,Joanne E. Russell,Rachel Urwin,Qing Zhang,Jiaji Zhou,Kerstin Zurth,Dominique A. Caugant,Ian M. Feavers,Mark Achtman,Brian G. Spratt +12 more
TL;DR: Multilocus sequence typing (MLST), which exploits the unambiguous nature and electronic portability of nucleotide sequence data for the characterization of microorganisms, can be applied to almost all bacterial species and other haploid organisms, including those that are difficult to cultivate.
Journal ArticleDOI
Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.
Paul R. Hunter,M A Gaston +1 more
TL;DR: An index of discrimination for typing methods is described, based on the probability of two unrelated strains being characterized as the same type, which may be used to compare typing methods and select the most discriminatory system.
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Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity.
Paul R. Hunter,M A Gaston +1 more