Journal ArticleDOI
High-level expression of human liver monoamine oxidase A in Pichia pastoris: comparison with the enzyme expressed in Saccharomyces cerevisiae.
TLDR
Data demonstrate significant advantages in the heterologous expression of human MAO-A in P. pastoris compared with the published S. cerevisiae system in higher expression level and in a higher level of homogeneity of the isolated enzyme.About:
This article is published in Protein Expression and Purification.The article was published on 2002-02-01. It has received 88 citations till now. The article focuses on the topics: Pichia pastoris & Flavin group.read more
Citations
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Journal ArticleDOI
Three-Dimensional Structure of Human Monoamine Oxidase a (Mao A): Relation to the Structures of Rat Mao a and Human Mao B
TL;DR: Data support the proposal that hMAO A involves a change from the dimeric to the monomeric form through a Glu-151 --> Lys mutation that is specific of hMAo A and put into question the use of MAO A from nonhuman sources in drug development for use in humans.
Journal ArticleDOI
Molecular and Mechanistic Properties of the Membrane-Bound Mitochondrial Monoamine Oxidases
TL;DR: This review summarizes the current structural and mechanistic information available that can be utilized in the development of future highly specific neuroprotectants and cardioprotectants.
Journal ArticleDOI
Demonstration of Isoleucine 199 as a Structural Determinant for the Selective Inhibition of Human Monoamine Oxidase B by Specific Reversible Inhibitors
Frantisek Hubalek,Claudia Binda,Ashraf A. Khalil,Min Li,Andrea Mattevi,Neal Castagnoli,Dale E. Edmondson +6 more
TL;DR: In this paper, the side chain of Ile-199 is rotated out of its normal conformation suggesting that Ile 199 is gating the substrate cavity, which is a determinant for the specificity of these reversible inhibitors.
Journal ArticleDOI
Monoamine oxidases as sources of oxidants in the heart.
Nina Kaludercic,Jeanne Mialet-Perez,Jeanne Mialet-Perez,Nazareno Paolocci,Angelo Parini,Angelo Parini,Fabio Di Lisa,Fabio Di Lisa +7 more
TL;DR: The role of MAO in catecholamine and serotonin clearance and cycling and cycling in relation to cardiac structure and function is discussed and both beneficial effects of their pharmacological or genetic inhibition along with potential adverse effects observed at baseline in MAO knockout mice are examined.
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Structural insights into the mechanism of amine oxidation by monoamine oxidases A and B
TL;DR: Compiling the recently available structural data on both enzymes with available mechanistic data as well as current NMR data on flavin systems supports the proposal that a polar nucleophilic mechanism for amine oxidation is the most consistent mechanistic scheme as compared with the single electron transfer mechanism.
References
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Journal ArticleDOI
cDNA cloning of human liver monoamine oxidase A and B: molecular basis of differences in enzymatic properties.
Bach Alfred,Nancy C. Lan,Deborah L. Johnson,Creed W. Abell,Michael E. Bembenek,Sau-Wah Kwan,Peter H. Seeburg,Jean C. Shih +7 more
TL;DR: Using oligonucleotide probes derived from three sequenced peptide fragments, isolated cDNA clones that encode the A and B forms of monoamine oxidase are isolated and the nucleotide sequences of these cDNAs are determined.
Journal ArticleDOI
The interpretation of collision‐induced dissociation tandem mass spectra of peptides
TL;DR: The fast atom bombardment (FABJ ionization mass spectrometry (MSJ) was used to obtain molecular weights of many nonvolatile molecules, including p e p tides comprising 10-20 amino acids as discussed by the authors.
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Rapid ‘de novo’ peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole/time‐of‐flight mass spectrometer
Andrej Shevchenko,Igor Chernushevich,Werner Ens,Kenneth G. Standing,Bruce A. Thomson,Matthias Wilm,Matthias Mann +6 more
TL;DR: A combination of the nanoelectrospray ion source, isotopic end labeling of peptides and a quadrupole/ time-of-flight instrument allows facile read-out of the sequences of tryptic peptides.
Journal ArticleDOI
The reactivity of flavoproteins with sulfite. Possible relevance to the problem of oxygen reactivity.
Vincent Massey,Franz Müller,Ross S. Feldberg,Marilyn Schuman,Patrick A. Sullivan,Larry G. Howell,Stephen G. Mayhew,Rowena G. Matthews,Gordon P. Foust +8 more
TL;DR: The correlation is made that those enzymes which react readily with sulfite are those which react catalytically with oxygen as a hydrogen acceptor; no sulfite reaction was found with flavoprotein dehydrogenases.