Journal ArticleDOI
High prevalence of Plamodium malariae infections in a Brazilian Amazon endemic area (Apiacás-Mato Grosso State) as detected by polymerase chain reaction.
Kézia K. G. Scopel,Cor Jesus Fernandes Fontes,Álvaro Cantini Nunes,M Fátima Horta,Érika Martins Braga +4 more
TLDR
The need of the development or use of a more accurate diagnosis method to distinguish between P. malariae and P. vivax is pointed to, which is particularly important in view of the fact that the choice of drug for the antimalarial therapy depends on the parasite species.About:
This article is published in Acta Tropica.The article was published on 2004-03-01. It has received 78 citations till now. The article focuses on the topics: Plasmodium malariae & Plasmodium vivax.read more
Citations
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Journal ArticleDOI
Malaria in Brazil: an overview
Joseli Oliveira-Ferreira,Marcus V. G. Lacerda,Patrícia Brasil,José Lázaro de Brito Ladislau,Pedro Luiz Tauil,Cláudio Tadeu Daniel-Ribeiro +5 more
TL;DR: Seropidemiological studies investigating the type of immune responses elicited in naturally-exposed populations to several malaria vaccine candidates in Brazilian populations have been providing important information on whether immune responses specific to these antigens are generated in natural infections and their immunogenic potential as vaccine candidates.
Journal ArticleDOI
Plasmodium malariae and Plasmodium ovale – the ‘bashful’ malaria parasites
TL;DR: Low-level infection seems to be common across malaria-endemic areas, often as complex mixed infections, and understanding these interactions could have an important influence on the deployment of interventions such as malaria vaccines.
Journal ArticleDOI
Malaria in Brazil, Colombia, Peru and Venezuela: current challenges in malaria control and elimination
Judith Recht,André M. Siqueira,Wuelton Marcelo Monteiro,Sonia M. Herrera,Sócrates Herrera,Marcus V. G. Lacerda +5 more
TL;DR: Challenges to continuing efforts to further decrease malaria incidence in this region include a significant increase in malaria cases in recent years in Venezuela, evidence of submicroscopic and asymptomatic infections, peri-urban malaria, gold mining-related malaria, malaria in pregnancy, glucose-6-phosphate dehydrogenase (G6PD) deficiency and primaquine use, and possible under-detection of Plasmodium malariae.
Journal ArticleDOI
Challenges for malaria elimination in Brazil
TL;DR: Challenges for malaria elimination addressed include the high prevalence of symptomless and submicroscopic infections, emerging anti-malarial drug resistance in P. falciparum and Plasmodium vivax and the lack of safe anti-relapse drugs, and the largely neglected burden of malaria in pregnancy.
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Amazonian malaria: Asymptomatic human reservoirs, diagnostic challenges, environmentally driven changes in mosquito vector populations, and the mandate for sustainable control strategies
Monica da Silva-Nunes,Marta Moreno,Jan E. Conn,Jan E. Conn,Dionicia Gamboa,Shira R. Abeles,Joseph M. Vinetz,Joseph M. Vinetz,Marcelo U. Ferreira +8 more
TL;DR: It is hypothesized that asymptomatic parasite carriage and massive environmental changes that affect vector abundance and behavior are major contributors to malaria transmission in epidemiologically diverse areas across the Amazon Basin.
References
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Journal ArticleDOI
Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections.
TL;DR: Genus- and species-specific sequences are present within the small subunit ribosomal RNA genes of the four human malaria parasites that have proven to be more sensitive and accurate than by routine diagnostic microscopy.
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How useful is PCR in the diagnosis of malaria
TL;DR: In most areas with malaria transmission, factors such as limited financial resources, persistent subclinical parasitaemia, inadequate laboratory infrastructures in the poorer, remote rural areas preclude PCR as a diagnostic method; even in affluent, non-endemic countries, PCR is not a suitable method for routine use.
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Identification of the four species of human malaria parasites by nested PCR that targets variant sequences in the small subunit rRNA gene
Masatsugu Kimura,Osamu Kaneko,Quing Liu,M. Zhou,Fumihiko Kawamoto,Yusuke Wataya,Shuzo Otani,Yoshiko Yamaguchi,Kazuyuki Tanabe +8 more
TL;DR: In this paper, a polymorphic region of the small subunit rRNA gene of the four human malaria parasite species was sequenced to see intraspecies variations and two new variant sequences were found in P. ovale and P. malariae.
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Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy.
TL;DR: A prospective 'blinded' comparison of diagnosis by conventional light microscopy and enzymatic amplification of the circumsporozoite gene extracted from dried spotted blood samples found P. falciparum and P. vivax infections to be mixed by the PCR/DNA probe systems.
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How prevalent is Plasmodium malariae in Rondônia, western Brazilian Amazon?
TL;DR: In this article, the authors compared results of Plasmodium species identification obtained with conventional on-site microscopy of Giemsa-stained thick smears (GTS) and a semi-nested polymerase chain reaction (PCR) in 96 malaria patients from Rondonia, Western Brazilian Amazon.