Histamine treatment induces rearrangements of orthogonal arrays of particles (OAPs) in human AQP4-expressing gastric cells
Monica Carmosino,Giuseppe Procino,Grazia Paola Nicchia,Roberta Mannucci,Jean-Marc Verbavatz,Renée Gobin,Maria Svelto,Giovanna Valenti +7 more
TLDR
Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport, the first evidence for short term rearrangement of OAPs in an established AQP 4-expressing cell line.Abstract:
To test the involvement of the water channel aquaporin (AQP)-4 in gastric acid physiology, the human gastric cell line (HGT)-1 was stably transfected with rat AQP4. AQP4 was immunolocalized to the basolateral membrane of transfected HGT-1 cells, like in native parietal cells. Expression of AQP4 in transfected cells increased the osmotic water permeability coefficient (Pf) from 2.02 +/- 0.3 x 10-4 to 16.37 +/- 0.5 x 10-4 cm/s at 20 degrees C. Freeze-fracture EM showed distinct orthogonal arrays of particles (OAPs), the morphological signature of AQP4, on the plasma membrane of AQP4-expressing cells. Quantitative morphometry showed that the density of OAPs was 2.5 +/- 0.3% under basal condition and decreased by 50% to 1.2 +/- 0.3% after 20 min of histamine stimulation, mainly due to a significant decrease of the OAPs number. Concomitantly, Pf decreased by approximately 35% in 20-min histamine-stimulated cells. Both Pf and OAPs density were not modified after 10 min of histamine exposure, time at which the maximal hormonal response is observed. Cell surface biotinylation experiments confirmed that AQP4 is internalized after 20 min of histamine exposure, which may account for the downregulation of water transport. This is the first evidence for short term rearrangement of OAPs in an established AQP4-expressing cell line.read more
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Omeprazole preserves the RER in chief cells and enhances re‑epithelialization of parietal cells with SOD and AQP‑4 up‑regulation in ethanol‑induced gastritis rats
TL;DR: The results of the present study suggest that omeprazole preserves the RER in chief cells and enhances parietal cell regeneration through its antioxidative property by exerting anti-inflammatory effects.
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Partitioning of aquaporin-4 water channel mRNA and protein in gastric glands.
TL;DR: The results suggest that cells harboring both protein and mRNA were observed in postnatal rats and in the pyloric region of the glandular stomach, during induced hypergastrinaemia represent a transition between those that bear only mRNA and those that are terminally differentiated, expressing proteins that are related to acid secretion.
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H3 receptor renal expression in normal and diabetic rats
TL;DR: H3R is expressed in the apical membrane by collecting duct cells in the kidney of rats and it is significantly increased in diabetic animals, suggesting its involvement in fluid homeostasis.
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Histamine and diabetic nephropathy: an up-to-date overview
TL;DR: The evidence collected on the role of histamine in kidney function together with its well-known pleiotropic action suggest that this amine may act simultaneously on glomerular hyperfiltration, tubular inflammation, fibrosis development and tubular hypertrophy.
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Functional coupling of chloride-proton exchanger ClC-5 to gastric H+,K+-ATPase.
Yuji Takahashi,Takuto Fujii,Kyosuke Fujita,Takahiro Shimizu,Taiga Higuchi,Yoshiaki Tabuchi,Hisato Sakamoto,Ichiro Naito,Koji Manabe,Shinichi Uchida,Sei Sasaki,Akira Ikari,Kazuhiro Tsukada,Hideki Sakai +13 more
TL;DR: The results suggest that ClC-5 and H+,K+-ATPase are functionally associated and that they may contribute to gastric acid secretion.
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