Journal ArticleDOI
Human sperm DNA integrity in normal and abnormal semen samples and its correlation with sperm characteristics
Alex C. Varghese,F. M. Bragais,Dyutiman Mukhopadhyay,S. Kundu,Manisha Pal,A.K. Bhattacharyya,Ashok Agarwal +6 more
TLDR
Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology, and assessment of sperm DNA status with AO staining may be helpful prior to ART.Abstract:
Reports indicate an increase in the incidence of DNA fragmentation in male factor infertility and its role in the outcome of assisted reproductive techniques (ART). However, reports are conflicting between the relationships of sperm DNA integrity with conventional semen parameters. We examined the relationship between conventional sperm parameters and DNA integrity using acridine orange (AO) test. The study included 373 patients and 28 fertile volunteers. DNA normality was compared with semen parameters between the patient and donor populations. Significant correlations were noted between DNA normality and sperm concentration (r = 0.18, P = 0.000), motility (r = 0.21, P = 0.0001), rapid motility (0.19, P = 0.000), normal morphology by World Health Organization (r = 0.15, P = 0.019) and head defects (r = -0.15, P = 0.023). A significant difference was noted in AO levels between donors and patients with asthenozoospermia (P = 0.002) and oligoasthenozoospermia (P = 0.001). A significant difference in DNA integrity was noted in samples having 30% normal morphology. A wide range of % DNA normality was observed in the patient group. Sperm assessment for DNA status using AO is reliable and shows good correlation with sperm count, motility and morphology. Assessment of sperm DNA status with AO staining may be helpful prior to ART.read more
Citations
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Journal ArticleDOI
Differential Production of Reactive Oxygen Species by Subsets of Human Spermatozoa
TL;DR: The results of this study indicate that there is significant cell-to-cell variation in ROS production in subsets of spermatozoa at different stages of maturation and that oxidative damage of mature spermatoza by ROS-producing immature spermatozosa during sperm migration from the seminiferous tubules to the epididymis may be an important cause of male infertility.
Journal ArticleDOI
Sperm DNA fragmentation index as a promising predictive tool for male infertility diagnosis and treatment management – meta-analyses
TL;DR: These meta-analyses demonstrate the sDF relevance in male infertility, suggesting a higher accuracy in detecting sperm function than conventional semen parameters.
Journal ArticleDOI
Poor sperm quality and advancing age are associated with increased sperm DNA damage in infertile men.
J Varshini,BS Srinag,Guruprasad Kalthur,Hanumanthappa Krishnamurthy,Pratap Kumar,Satish Rao,Satish Kumar Adiga +6 more
TL;DR: Age‐related increase in sperm DNA damage and association of the same with varicocele and alcohol consumption are demonstrated, and poor sperm characteristics in the ejaculate are associated with increased sperm DNADamage.
Journal ArticleDOI
Sperm DNA damage or progressive motility: which one is the better predictor of fertilization in vitro?
Luke Simon,Sheena E.M. Lewis +1 more
TL;DR: This study shows that fertilization rates are directly dependent upon both sperm progressive motility and DNA fragmentation, but sperm DNA fragmentation is a much stronger test.
Journal ArticleDOI
Effect of smoking on semen quality, FSH, testosterone level, and CAG repeat length in androgen receptor gene of infertile men in an Indian city
Amrita Mitra,Baidyanath Chakraborty,Dyutiman Mukhopadhay,Manisha Pal,Sanjit Mukherjee,Samir Banerjee,Keya Chaudhuri +6 more
TL;DR: The study suggested that smoking is associated with altered semen quality, endocrine hormonal status, and number of CAG repeats in the AR gene.
References
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Journal ArticleDOI
Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Donald P. Evenson,Lorna K. Jost,D Marshall,M J Zinaman,E D Clegg,K Purvis,P. De Angelis,O P Claussen +7 more
TL;DR: Based on logistic regression, spermatozoa with denatured DNA (cells outside the main population, COMP alpha t) were the best predictor for whether a couple would not achieve pregnancy.
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Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques.
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Association of In Vitro Fertilization with Beckwith-Wiedemann Syndrome and Epigenetic Alterations of LIT1 and H19
TL;DR: The first evidence, to the knowledge, is reported that ART is associated with a human overgrowth syndrome-namely, Beckwith-Wiedemann syndrome (BWS), similar to the large offspring syndrome reported in ruminants.
Journal ArticleDOI
Relation of mammalian sperm chromatin heterogeneity to fertility.
TL;DR: Flow cytometry of heated sperm nuclei revealed a significant decrease in resistance to in situ denaturation of spermatozoal DNA in samples from bulls, mice, and humans of low or questionable fertility when compared with others of high fertility.
Journal ArticleDOI
Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa
TL;DR: Human spermatozoa exposed to increasing levels of oxidative stress achieved through the stimulation of endogenous oxidant generation with NADPH or direct exposure to hydrogen peroxide exhibited significantly elevated levels of DNA damage and yet continued to express an enhanced capacity for sperm-oocyte fusion.