Journal ArticleDOI
Hybridization of nucleic acids directly in agarose gels.
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TLDR
The method requires less manipulation than other procedures; it is extremely rapid, sensitive, and inexpensive; these attributes make this procedure a valuable alternative or supplement to the commonly used methods for visualization by hybridization of nucleic acids separated on agarose gels.About:
This article is published in Analytical Biochemistry.The article was published on 1983-06-01. It has received 171 citations till now. The article focuses on the topics: Nucleic acid quantitation & Gel electrophoresis of nucleic acids.read more
Citations
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Journal ArticleDOI
Hybridization of nucleic acids immobilized on solid supports
Judy Meinkoth,Geoffrey M. Wahl +1 more
Book
DNA fingerprinting in plants: principles, methods, and applications
TL;DR: Amin et al. as discussed by the authors used radioisotopes to measure the diversity of microsatellites in the field of plant DNA collection and preservation of plant Tissue in the Field.
Book ChapterDOI
Oligonucleotide probes for the screening of recombinant DNA libraries
Wallace Rb,Miyada Cg +1 more
TL;DR: This chapter deals with procedures for the use of oligonucleotides as hybridization probes including probe design, labeling, and hybridization to colonies, phage plaques, DNA, and RNA.
Journal ArticleDOI
DNA finger printing by oligonucleotide probes specific for simple repeats.
TL;DR: Oligonucleotide probes specific for variations of the GACTA simple repeats have been designed and hybridized to a panel of human DNAs digested with various restriction enzymes, and individual-specific hybridization patterns (“DNA fingerprints”) can be established.
Journal ArticleDOI
Multicentre experience in the treatment of burns with autologous and allogenic cultured epithelium, fresh or preserved in a frozen state
M. De Luca,Enrico Albanese,Sergio Bondanza,M. Megna,L. Ugozzoli,F. Molina,Ranieri Cancedda,P.L. Santi,M. Bormioli,M. Stella,G. Magliacani +10 more
TL;DR: Clinical results obtained from a multicentre experience of the use of autologous and allogenic cultured human epidermal cells in the treatment of partial and full skin thickness burns are described.
References
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Journal ArticleDOI
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
Journal ArticleDOI
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I
TL;DR: Labeled DNAs (and restriction endonuclease fragments derived from them) are useful probes for detecting rare homologous sequences by in situ hybridization and reassociation kinetic analysis.
Journal ArticleDOI
Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification.
TL;DR: Extension of this method to concentration and purification of other viruses and nucleic acids, as well as some preliminary mechanistic studies, are discussed.
Journal ArticleDOI
The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper
Gale E. Smith,Max D. Summers +1 more
TL;DR: DNA fragments from 5% polyacrylamide gels were efficiently blotted after 36 h onto nitrocellulose filters using bidirectional transfer and were shown to be efficient substrates for homologous [32P]DNA probes.