Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
Reads0
Chats0
TLDR
It is suggested that guard cells treated with ABA may close the stomata via a pathway with H(2)O(2), which may be an intermediate in ABA signaling, and H( 2)OAbstract:
One of the most important functions of the plant hormone abscisic acid (ABA) is to induce stomatal closure by reducing the turgor of guard cells under water deficit. Under environmental stresses, hydrogen peroxide (H 2 O 2 ), an active oxygen species, is widely generated in many biological systems. Here, using an epidermal strip bioassay and laser-scanning confocal microscopy, we provide evidence that H 2 O 2 may function as an intermediate in ABA signaling in Vicia faba guard cells. H 2 O 2 inhibited induced closure of stomata, and this effect was reversed by ascorbic acid at concentrations lower than 10 −5 m. Further, ABA-induced stomatal closure also was abolished partly by addition of exogenous catalase (CAT) and diphenylene iodonium (DPI), which are an H 2 O 2 scavenger and an NADPH oxidase inhibitor, respectively. Time course experiments of single-cell assays based on the fluorescent probe dichlorofluorescein showed that the generation of H 2 O 2 was dependent on ABA concentration and an increase in the fluorescence intensity of the chloroplast occurred significantly earlier than within the other regions of guard cells. The ABA-induced change in fluorescence intensity in guard cells was abolished by the application of CAT and DPI. In addition, ABA microinjected into guard cells markedly induced H 2 O 2 production, which preceded stomatal closure. These effects were abolished by CAT or DPI micro-injection. Our results suggest that guard cells treated with ABA may close the stomata via a pathway with H 2 O 2 production involved, and H 2 O 2 may be an intermediate in ABA signaling.read more
Citations
More filters
Journal ArticleDOI
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction
Klaus Apel,Heribert Hirt +1 more
TL;DR: The mechanisms of ROS generation and removal in plants during development and under biotic and abiotic stress conditions are described and the possible functions and mechanisms for ROS sensing and signaling in plants are compared with those in animals and yeast.
Journal ArticleDOI
Reactive oxygen species homeostasis and signalling during drought and salinity stresses
TL;DR: An overview of ROS homeostasis and signalling in response to drought and salt stresses is provided and the current understanding of ROS involvement in stress sensing, stress signalling and regulation of acclimation responses is discussed.
Journal ArticleDOI
Cell Signaling during Cold, Drought, and Salt Stress
TL;DR: Low temperature, drought, and high salinity are common stress conditions that adversely affect plant growth and crop production and understand the cellular and molecular responses of plants to environmental stress.
Journal ArticleDOI
Abscisic Acid Signaling in Seeds and Seedlings
TL;DR: Abscisic acid regulates many agronomically important aspects of plant development, including the synthesis of seed storage proteins and lipids, the promotion of seed desiccation tolerance and dormancy, and the inhibition of the phase transitions from embryonic to germinative growth and from.
Journal ArticleDOI
Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context
Christine H. Foyer,Graham Noctor +1 more
TL;DR: It is proposed that the oxidative stress syndrome would be more usefully described as 'oxidative signalling', that is, an important and critical function associated with the mechanisms by which plant cells sense the environment and make appropriate adjustments to gene expression, metabolism and physiology.
References
More filters
Journal ArticleDOI
The Origin of the Oxidative Burst in Plants
TL;DR: An alternative simple and rapid mechanism thus exists for the generation of H2O2 which does not require such multiple proteinaceous components.
Journal ArticleDOI
Abscisic acid induces oscillations in guard-cell cytosolic free calcium that involve phosphoinositide-specific phospholipase C.
I. Staxen,Christophe Pical,L. T. Montgomery,Julie E. Gray,Alistair M. Hetherington,Martin R. McAinsh +5 more
TL;DR: It is shown that ABA induces oscillations in guard-cell [Ca2+]cyt, and a role for PI-PLC in the generation of ABA-induced oscillations is suggested, which point toward the involvement ofscillations in [Ca 2+] Cyt in the maintenance of stomatal aperture by ABA.
Journal ArticleDOI
Ca2+ channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and abscisic acid
TL;DR: Results identify a Ca(2+)-selective channel at the guard cell plasma membrane that can account for Ca( 2+) influx and increases in [Ca( 2+)](i) triggered by voltage and ABA, and they imply a close physical coupling at the plasma membrane between ABA perception and Ca (2+) channel control.
Journal ArticleDOI
Role of Calcium in Signal Transduction of Commelina Guard Cells.
TL;DR: The endogenous growth regulator abscisic acid elevated cytosolic Ca2+ levels in a minority of cells investigated, even though stomatal closure always occurred.
Journal ArticleDOI
Current advances in abscisic acid action and signalling.
Jérôme Giraudat,François Parcy,Nathalie Bertauche,Françoise Gosti,Jeffrey Leung,Peter-Christian Morris,Michelle Bouvier-Durand,Nicole Vartanian +7 more
TL;DR: The characterization of deficient mutants has clarified the ABA biosynthetic pathway in higher plants, and characterization of mutants defective in ABA responsiveness, and molecular cloning of the corresponding loci has proven a powerful approach to dissect the molecular nature of ABA signalling cascades.