Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
TLDR
It is suggested that guard cells treated with ABA may close the stomata via a pathway with H(2)O(2), which may be an intermediate in ABA signaling, and H( 2)OAbstract:
One of the most important functions of the plant hormone abscisic acid (ABA) is to induce stomatal closure by reducing the turgor of guard cells under water deficit. Under environmental stresses, hydrogen peroxide (H 2 O 2 ), an active oxygen species, is widely generated in many biological systems. Here, using an epidermal strip bioassay and laser-scanning confocal microscopy, we provide evidence that H 2 O 2 may function as an intermediate in ABA signaling in Vicia faba guard cells. H 2 O 2 inhibited induced closure of stomata, and this effect was reversed by ascorbic acid at concentrations lower than 10 −5 m. Further, ABA-induced stomatal closure also was abolished partly by addition of exogenous catalase (CAT) and diphenylene iodonium (DPI), which are an H 2 O 2 scavenger and an NADPH oxidase inhibitor, respectively. Time course experiments of single-cell assays based on the fluorescent probe dichlorofluorescein showed that the generation of H 2 O 2 was dependent on ABA concentration and an increase in the fluorescence intensity of the chloroplast occurred significantly earlier than within the other regions of guard cells. The ABA-induced change in fluorescence intensity in guard cells was abolished by the application of CAT and DPI. In addition, ABA microinjected into guard cells markedly induced H 2 O 2 production, which preceded stomatal closure. These effects were abolished by CAT or DPI micro-injection. Our results suggest that guard cells treated with ABA may close the stomata via a pathway with H 2 O 2 production involved, and H 2 O 2 may be an intermediate in ABA signaling.read more
Citations
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Freezing tolerance and antioxidant activity in potato microplants induced by abscisic acid treatment
TL;DR: The potential of ABA in the induction of freezing tolerance in microplants transferred to soil was demonstrated and Responses to ABA were associated with the antioxidant system and H2O2 content in the induction of freezingolerance in potato.
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Transcriptome-wide characterization of candidate genes for improving the water use efficiency of energy crops grown on semiarid land
Yangyang Fan,Qian Wang,Lifang Kang,Wei Liu,Qin Xu,Shilai Xing,Chengcheng Tao,Zhihong Song,Caiyun Zhu,Cong Lin,Juan Yan,Jianqiang Li,Tao Sang +12 more
TL;DR: A matrix correlation analysis between transcriptome-wide expression levels and water use efficiency of Miscanthus lutarioriparius identified candidate genes facilitating adaptation of the energy crop to semiarid marginal land.
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Molecular cloning, characterisation and expression of two catalase genes from peach
Francesca Bagnoli,Susanna Danti,Valentina Magherini,Radiana Cozza,Anna Maria Innocenti,M. L. Racchi +5 more
TL;DR: Two cDNA clones encoding catalase (Cat1 and Cat2) from peach were identified, that show homologies to other plant catalases, suggesting a role for Cat1 in photorespiration and for Cat2 in stress responses.
Book ChapterDOI
Abscisic Acid Signal Transduction in Stomatal Responses
TL;DR: ABA plays major roles in the regulation of several important plant processes, including for plant acclimation to drought, cold, and salinity, for development of seed dormancy, and for inhibition of seed germination.
Journal ArticleDOI
H2O2 Inhibits ABA-Signaling Protein Phosphatase HAB1
Madhuri Sridharamurthy,Madhuri Sridharamurthy,Amanda Kovach,Yang Zhao,Jian-Kang Zhu,H. Eric Xu,H. Eric Xu,Kunchithapadam Swaminathan,Karsten Melcher +8 more
TL;DR: Using a reconstituted system, it is demonstrated that H2O2 reversibly prevents the protein phosphatase HAB1, a key component of the core ABA-signaling pathway, from inhibiting its main target in guard cells, SnRK2.6/OST1 kinase.
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