Hydrogen Peroxide Is Involved in Abscisic Acid-Induced Stomatal Closure in Vicia faba
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It is suggested that guard cells treated with ABA may close the stomata via a pathway with H(2)O(2), which may be an intermediate in ABA signaling, and H( 2)OAbstract:
One of the most important functions of the plant hormone abscisic acid (ABA) is to induce stomatal closure by reducing the turgor of guard cells under water deficit. Under environmental stresses, hydrogen peroxide (H 2 O 2 ), an active oxygen species, is widely generated in many biological systems. Here, using an epidermal strip bioassay and laser-scanning confocal microscopy, we provide evidence that H 2 O 2 may function as an intermediate in ABA signaling in Vicia faba guard cells. H 2 O 2 inhibited induced closure of stomata, and this effect was reversed by ascorbic acid at concentrations lower than 10 −5 m. Further, ABA-induced stomatal closure also was abolished partly by addition of exogenous catalase (CAT) and diphenylene iodonium (DPI), which are an H 2 O 2 scavenger and an NADPH oxidase inhibitor, respectively. Time course experiments of single-cell assays based on the fluorescent probe dichlorofluorescein showed that the generation of H 2 O 2 was dependent on ABA concentration and an increase in the fluorescence intensity of the chloroplast occurred significantly earlier than within the other regions of guard cells. The ABA-induced change in fluorescence intensity in guard cells was abolished by the application of CAT and DPI. In addition, ABA microinjected into guard cells markedly induced H 2 O 2 production, which preceded stomatal closure. These effects were abolished by CAT or DPI micro-injection. Our results suggest that guard cells treated with ABA may close the stomata via a pathway with H 2 O 2 production involved, and H 2 O 2 may be an intermediate in ABA signaling.read more
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References
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Journal ArticleDOI
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control
Graham Noctor,Christine H. Foyer +1 more
TL;DR: A detailed account of current knowledge of the biosynthesis, compartmentation, and transport of these two important antioxidants, with emphasis on the unique insights and advances gained by molecular exploration are provided.
Journal ArticleDOI
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
TL;DR: It is reported here that H2O2 from this oxidative burst not only drives the cross-linking of cell wall structural proteins, but also functions as a local trigger of programmed death in challenged cells and as a diffusible signal for the induction in adjacent cells of genes encoding cellular protectants.
Journal ArticleDOI
Superoxide dismutase and stress tolerance
TL;DR: Prospects for Stress Tolerance through Genetic Engineering of SOD and MnSOD Overexpression are surveyed, and the Mechanism of Sod Regulation is studied.
Journal ArticleDOI
Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Zhen-Ming Pei,Yoshiyuki Murata,Gregor Benning,Sébastien Thomine,Birgit Klüsener,Gethyn J. Allen,Erwin Grill,Julian I. Schroeder +7 more
TL;DR: Activation of Ca2+-permeable channels in the plasma membrane of Arabidopsis guard cells by hydrogen peroxide indicates that ABA-induced H2O2 production and the H 2O 2-activated Ca2-activated channels are important mechanisms for A BA-induced stomatal closing.
Journal ArticleDOI
Oxygen Stress and Superoxide Dismutases
TL;DR: The accumulation of dioxygen in Earth's atmosphere allowed for the evolution of aerobic organisms that use O2 as the terminal electron acceptor, thus providing a higher yield of energy compared with fermentation and anaerobic respiration.