Open AccessJournal Article
Hydroxynitrile lyases: At the interface of biology and chemistry
TLDR
In chemical industries, hydroxynitrile lyase is used as an important industrial biocatalyst for the synthesis of chiral cyanohydrins by exploiting the reversible enzymatic reaction.Abstract:
Hydroxynitrile lyases are the versatile group of enzymes,which play a significant defensive role in plant system against microbial attack. In chemical industries, hydroxynitrile lyase is used as an important industrial biocatalyst for the synthesis of chiral cyanohydrins by exploiting the reversible enzymatic reaction. Cyanohydrins are biologically active compounds used in synthesis of β-amino alcohols, a-hydroxy ketones anda-hydroxy acids, which have importance as fine chemicals, Pharmaceuticals and agrochemicals. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various ydroxynitrile lyases. Many recombinant hydroxynitrile lyases have been expressed in Escherichia coli, Saccharomyces cerevisiae and Pichia pastoris.read more
Citations
More filters
Dissertation
In vitro and In silico Biocatalytic Studies of a Plant Hydroxynitrile lyase
TL;DR: In order to explore the use of AtHNL’s catalytic ability toward other substrates especially with respect to nitroaldol synthesis, the binding ability of various -nitroalcohol analogs is studied by docking them into the AtH NL active site.
Dissertation
Screening, purification and characterisation of an active Hydroxynitrile Lyase (Nitrilase) from indigenous South African Plants
TL;DR: The experimental data obtained alluded to the notion that both KsHNL and SbHNL enzymes may contain the cysteine and serine residues next to their active sites and that a histidine residue may be involved in the catalytic activities of both the isolated Ks hNL enzymes and one of the Sb HNL enzymes.
References
More filters
Journal ArticleDOI
The BioCassava plus program: biofortification of cassava for sub-Saharan Africa.
Richard T. Sayre,John R. Beeching,Edgar B. Cahoon,Chiedozie Egesi,Claude M. Fauquet,John K. Fellman,Martin A. Fregene,Wilhelm Gruissem,Sally Mallowa,Mark J. Manary,B.B. Maziya-Dixon,Ada Mbanaso,Daniel P. Schachtman,Dimuth Siritunga,Nigel J. Taylor,Hervé Vanderschuren,Peng Zhang +16 more
TL;DR: Results from field trials in Puerto Rico, the first confined field Trials in Nigeria, and ex ante impact analyses support the efficacy of using transgenic strategies for the biofortification of cassava.
Journal ArticleDOI
Potential and capabilities of hydroxynitrile lyases as biocatalysts in the chemical industry
TL;DR: Recently, protein engineering in combination with in silico methods gave rise to the development of a tailor-made HNL for large-scale manufacturing of a specific target cyanohydrin.
Journal ArticleDOI
How to overcome limitations in biotechnological processes - examples from hydroxynitrile lyase applications
TL;DR: By focusing on the hydroxynitrile lyase-catalysed synthesis of enantiopure cyanohydrins, this work gives an overview of strategies to improve commercially utilized enzymes and to suppress non-enzymatic reactions with particular emphasis on the necessity to combine approaches from different fields, such as enzyme engineering and reaction engineering.
Journal ArticleDOI
Study of the Early Events Leading to Cassava Root Postharvest Deterioration
TL;DR: Physiological and biochemical changes occurring 3 or 4 h after cassava root detachment were observed along with a correspondent increase in tissue cytoplasmic singlet oxygen presence using radical-specific fluorescent imaging of tissue samples, likely that these findings have significant implications to help understand and assist in dissection of the early events leading to the postharvest deterioration of cassavaroot.
Journal ArticleDOI
Nitrile Metabolizing Enzymes in Biocatalysis and Biotransformation.
TL;DR: A number of opportunities and challenges of nitrile metabolizing enzymes in bioprocess development for the production of bulk and fine chemicals are discussed.