Identification of Genes Involved in the Response of Arabidopsis to Simultaneous Biotic and Abiotic Stresses
Reads0
Chats0
TLDR
The transcriptome response of Arabidopsis to concurrent water deficit and infection with the plant-parasitic nematode Heterodera schachtii and candidate genes with potential roles in controlling the response to multiple stresses were selected and functionally characterized.Abstract:
In field conditions, plants may experience numerous environmental stresses at any one time. Research suggests that the plant response to multiple stresses is different from that for individual stresses, producing nonadditive effects. In particular, the molecular signaling pathways controlling biotic and abiotic stress responses may interact and antagonize one another. The transcriptome response of Arabidopsis (Arabidopsis thaliana) to concurrent water deficit (abiotic stress) and infection with the plant-parasitic nematode Heterodera schachtii (biotic stress) was analyzed by microarray. A unique program of gene expression was activated in response to a combination of water deficit and nematode stress, with 50 specifically multiple-stress-regulated genes. Candidate genes with potential roles in controlling the response to multiple stresses were selected and functionally characterized. RAPID ALKALINIZATION FACTOR-LIKE8 (AtRALFL8) was induced in roots by joint stresses but conferred susceptibility to drought stress and nematode infection when overexpressed. Constitutively expressing plants had stunted root systems and extended root hairs. Plants may produce signal peptides such as AtRALFL8 to induce cell wall remodeling in response to multiple stresses. The methionine homeostasis gene METHIONINE GAMMA LYASE (AtMGL) was up-regulated by dual stress in leaves, conferring resistance to nematodes when overexpressed. It may regulate methionine metabolism under conditions of multiple stresses. AZELAIC ACID INDUCED1 (AZI1), involved in defense priming in systemic plant immunity, was down-regulated in leaves by joint stress and conferred drought susceptibility when overexpressed, potentially as part of abscisic acid-induced repression of pathogen response genes. The results highlight the complex nature of multiple stress responses and confirm the importance of studying plant stress factors in combination.read more
Citations
More filters
Journal ArticleDOI
LSU network hubs integrate abiotic and biotic stress responses via interaction with the superoxide dismutase FSD2.
Antoni Garcia-Molina,Melina Altmann,Angela Alkofer,Petra Epple,Jeffery L. Dangl,Pascal Falter-Braun +5 more
TL;DR: It is demonstrated that reduced LSU levels cause a moderately enhanced disease susceptibility in plants exposed to abiotic stresses such as nutrient deficiency, high salinity, or heavy metal toxicity, whereas LSU1 overexpression confers significant disease resistance in several of these conditions.
Book ChapterDOI
The Response of Plants to Simultaneous Biotic and Abiotic Stress
TL;DR: This chapter examines the mechanisms by which plants respond to simultaneous biotic and abiotic stresses, highlighting the effects on agriculture, and in particular rice.
Journal ArticleDOI
Cultivar-Dependent Responses of Eggplant (Solanum melongena L.) to Simultaneous Verticillium dahliae Infection and Drought.
Eleni Tani,Dimosthenis Kizis,Emilia Markellou,Ioannis E. Papadakis,Dimitra Tsamadia,Georgios Leventis,Despoina Makrogianni,Ioannis Karapanos +7 more
TL;DR: Combined stress affects significantly plants responses compared to the application of individual stresses alone and that these responses are cultivar dependent, showing a clear discrimination between the two cultivars in response to simultaneous V. dahliae infection and drought.
Journal ArticleDOI
A rust fungal effector binds plant DNA and modulates transcription.
Bulbul Ahmed,Karen Cristine Gonçalves dos Santos,Ingrid Benerice Sanchez,Ingrid Benerice Sanchez,Benjamin Petre,Benjamin Petre,Cécile Lorrain,Mélodie B. Plourde,Sébastien Duplessis,Isabel Desgagné-Penix,Hugo Germain +10 more
TL;DR: The results suggest that Mlp124478 exerts a virulence activity and binds the TGA1a promoter to suppress genes induced in response to pathogen infection.
Journal ArticleDOI
Arabidopsis thaliana rapid alkalinization factor 1-mediated root growth inhibition is dependent on calmodulin-like protein 38.
Wellington F Campos,Keini Dressano,Paulo H. O. Ceciliato,Juan Carlos Guerrero-Abad,Aparecida Leonir da Silva,Celso S. Fiori,Amanda Morato do Canto,Tábata Bergonci,Lucas Alves Neubus Claus,Marcio C. Silva-Filho,Daniel S. Moura +10 more
TL;DR: A calmodulin-like protein that binds AtRALF1, is essential for root growth inhibition, and has no role in AtralF1 alkalinization response is described.
References
More filters
Journal ArticleDOI
Exploration, normalization, and summaries of high density oligonucleotide array probe level data
Rafael A. Irizarry,Bridget G. Hobbs,Francois Collin,Yasmin Beazer-Barclay,Kristen J. Antonellis,Uwe Scherf,Terence P. Speed +6 more
TL;DR: There is no obvious downside to using RMA and attaching a standard error (SE) to this quantity using a linear model which removes probe-specific affinities, and the exploratory data analyses of the probe level data motivate a new summary measure that is a robust multi-array average (RMA) of background-adjusted, normalized, and log-transformed PM values.
Journal ArticleDOI
MYB transcription factors in Arabidopsis
TL;DR: The elucidation ofMYB protein function and regulation that is possible in Arabidopsis will provide the foundation for predicting the contributions of MYB proteins to the biology of plants in general.
Journal ArticleDOI
Understanding plant responses to drought — from genes to the whole plant
TL;DR: Attention is drawn to the perception and signalling processes (chemical and hydraulic) of water deficits, which are essential for a holistic understanding of plant resistance to stress, which is needed to improve crop management and breeding techniques.
Journal ArticleDOI
Abiotic stress, the field environment and stress combination
TL;DR: Tolerance to a combination of different stress conditions, particularly those that mimic the field environment, should be the focus of future research programs aimed at developing transgenic crops and plants with enhanced tolerance to naturally occurring environmental conditions.
Journal ArticleDOI
Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray.
Motoaki Seki,Mari Narusaka,Junko Ishida,Tokihiko Nanjo,Miki Fujita,Youko Oono,Asako Kamiya,Maiko Nakajima,Akiko Enju,Tetsuya Sakurai,Masakazu Satou,Kenji Akiyama,Teruaki Taji,Kazuko Yamaguchi-Shinozaki,Piero Carninci,Jun Kawai,Yoshihide Hayashizaki,Kazuo Shinozaki +17 more
TL;DR: A full-length cDNA microarray containing approximately 7000 independent, full- length cDNA groups is prepared to analyse the expression profiles of genes under drought, cold (low temperature) and high-salinity stress conditions over time, suggesting that various transcriptional regulatory mechanisms function in the drought,cold or high- salinity stress signal transduction pathways.
Related Papers (5)
The interaction of plant biotic and abiotic stresses: from genes to the field
Nicky J. Atkinson,Peter E. Urwin +1 more