Identification of sequences involved in the transcriptional control of a Drosophila heat-shock gene.
Victor G. Corces,Angel Pellicer +1 more
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TLDR
It is proposed that there are two additional regulatory elements besides the consensus sequence which must be present for proper temperature-dependent transcription to occur and either one of these elements may serve at any one time to ensure transcription.About:
This article is published in Journal of Biological Chemistry.The article was published on 1984-12-10 and is currently open access. It has received 32 citations till now. The article focuses on the topics: CAAT box & Promoter.read more
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Journal ArticleDOI
RNA splicing is interrupted by heat shock and is rescued by heat shock protein synthesis
H. Joseph Yost,Susan Lindquist +1 more
TL;DR: It is suggested that the disruption of intron processing contributes to heat- induced lethality and developmental abnormalities and that one function of the heat shock proteins is to protect processing from heat-induced disruption.
Journal ArticleDOI
Streptogramin-based gene regulation systems for mammalian cells.
Martin Fussenegger,Rowan P. Morris,Cornelia Fux,Markus Rimann,Beryl von Stockar,Charles J. Thompson,James E. Bailey +6 more
TL;DR: The streptogramin-based expression technology was functionally compatible with the TetOFF system, thus enabling the selective use of different antibiotics to independently control two different gene activities in the same cell.
Journal ArticleDOI
Human HSP70 promoter contains at least two distinct regulatory domains.
TL;DR: To determine whether transcription is mediated through a single or multiple genetic elements, the sequences upstream of the transcription start site of the human HSP70 gene are dissected by constructing chimeric genes retaining variable amounts of 5' flanking regions fused to the bacterial gene encoding chloramphenicol acetyltransferase.
Human HSP70 promoter contains at least two distinct regulatory domains (heat shock induction/cadmium induction/serum stimulation/DNA-mediated gene transfer)
TL;DR: The human HSP70 gene is induced by a wide range of physiological stresses, including exposure to heat shock and heavy metals, or under nonstress conditions, such as in response to serum stimulation.
Journal ArticleDOI
The impact of mammalian gene regulation concepts on functional genomic research, metabolic engineering, and advanced gene therapies
TL;DR: This review presents a detailed analysis of currently available mammalian gene regulation systems and document progress that has pioneered the use of such systems in various aspects of human therapy.
References
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Book ChapterDOI
Sequencing end-labeled DNA with base-specific chemical cleavages.
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: The chapter presents techniques for producing discrete DNA fragments, end-labeling DNA, segregating end- labeled fragments, extracting DNA from gels, and the protocols for partially cleaving it at specific bases using the chemical reactions.
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Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
TL;DR: A simple and rapid method for transferring RNA from agarose gels to nitrocellulose paper for blot hybridization has been developed, allowing removal of the hybridized probes and rehybridization of the RNA blots without loss of sensitivity.
Journal ArticleDOI
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells
TL;DR: The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase to the aprt+ phenotype by means of DNA-mediated gene transfer is demonstrated.
Journal ArticleDOI
The 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I
TL;DR: Many specific sites in Drosophila chromatin are hypersensitive to DNase I and the positions of such sites were mapped along the regions of the genome coding for two heat shock proteins.
Journal ArticleDOI
A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene
TL;DR: It seems that the upstream element of the hsp 70 promoter is analogous to that of other promoters, but is only functional in heat-shocked cells.