Journal ArticleDOI
A regulatory upstream promoter element in the Drosophila Hsp 70 heat-shock gene
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It seems that the upstream element of the hsp 70 promoter is analogous to that of other promoters, but is only functional in heat-shocked cells.About:
This article is published in Cell.The article was published on 1982-09-01. It has received 897 citations till now. The article focuses on the topics: COS cells & Promoter.read more
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Regulation of inducible and tissue-specific gene expression
TL;DR: A number of cases of inducible and tissue-specific gene expression involve the activation of preexisting transcription factors, rather than the synthesis of new proteins, which may involve covalent modification of the protein or an allosteric change in its structure.
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An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
Sean Munro,Hugh R.B. Pelham +1 more
TL;DR: A cDNA clone is characterized that encodes a protein related to the 70 kd heat shock protein, but is expressed in normal rat liver, and it is identical with two previously described proteins: GRP78, whose synthesis is induced by glucose starvation, and BiP, which is found bound to immunoglobulin heavy chains in pre-B cells.
Journal ArticleDOI
Heat shock factors: integrators of cell stress, development and lifespan
TL;DR: These unexpected observations have uncovered complex layers of post-translational regulation of HSFs that integrate the metabolic state of the cell with stress biology, and in doing so control fundamental aspects of the health of the proteome and ageing.
Journal ArticleDOI
Maize polyubiquitin genes: structure, thermal perturbation of expression and transcript splicing, and promoter activity following transfer to protoplasts by electroporation.
TL;DR: Two genomic clones (λUbi-1 and λUBI-2) encoding the highly conserved 76 amino acid protein ubiquitin have been isolated from maize and sequence analysis shows that both genes contain seven contiguous direct repeats of the protein coding region in a polyprotein conformation.
Journal ArticleDOI
Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins
TL;DR: The enzyme that carries out RNA synthesis provides the cell with the means to adjust the patterns of transcription in response to environmental and developmental signals, and the presence of promoter-specific transcription factors enables other components of the transcriptional machinery, including the RNA polymerase, to initiate transcription with selectivity and accuracy.
References
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Journal ArticleDOI
A new method for sequencing DNA
Allan M. Maxam,Walter Gilbert +1 more
TL;DR: Reactions that cleave DNA preferentially at guanines, at adenines,At cytosines and thymines equally, and at cytosine alone are described.
Journal ArticleDOI
SV40-transformed simian cells support the replication of early SV40 mutants.
TL;DR: Three transformed lines of simian cells were established and found to contain T antigen; retain complete permissiveness for lytic growth of SV40; support the replication of tsA209 virus at 40 degrees C; and support the replicate of pure populations of SV 40 mutants with deletions in the early region.
Journal ArticleDOI
Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
Arnold J. Berk,Phillip A. Sharp +1 more
TL;DR: A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.
Journal ArticleDOI
Rapid and efficient cosmid cloning.
D. Ish-Horowicz,Julian F. Burke +1 more
TL;DR: The method eliminates the need to size the insert DNA fragments and prevents formation of clones containing short or multiple inserts and a rapid and efficient method for preparing plasmid and cosmid DNA is described.
Journal ArticleDOI
Expression of a β-globin gene is enhanced by remote SV40 DNA sequences
TL;DR: The activation of genes by specific enhancer elements seems to be a widespread mechanism that may be used for the regulation of gene expression and defines a class of DNA elements with a mode of action that has not been heretofore described.
Related Papers (5)
A Drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene.
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