Journal ArticleDOI
Identification of the regulatory subunit of a cAMP-dependent protein kinase in Dictyosteliumdiscoideum
TLDR
In this paper, the occurrence of a cytosolic cAMP-binding protein of an approximate molecular weight of 41,000 daltons was monitored in vegetative and developing amoebae of Dictyostelium discoideum by the use of the photoaffinity probe (32P) 8N3-cAMP.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 1982-04-14. It has received 29 citations till now. The article focuses on the topics: Dictyostelium discoideum & Protein kinase A.read more
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Journal ArticleDOI
Multiple roles for cAMP-dependent protein kinase during Dictyostelium development.
Adrian J. Harwood,N. A. Hopper,Marie-Noëlle Simon,S. Bouzid,Michel Véron,Jeffrey G. Williams +5 more
TL;DR: It is found that a number of postaggregative genes are not expressed in PKA- cells, even when they are allowed to synergise with normal cells, which suggests that PKA may be required during the late, multicellular stages of development.
Journal ArticleDOI
Role of cAMP-dependent protein kinase in controlling aggregation and postaggregative development in Dictyostelium.
Sandra K.O. Mann,Jason M Brown,Celia Briscoe,Carole A. Parent,Geoffrey S. Pitt,Peter N. Devreotes,Richard A. Firtel +6 more
TL;DR: The results indicate that pkacat- cells lack multiple, essential aggregation-stage functions as the mound forms, and new, essential regulatory roles for PKA are identified in mediating multicellular development.
Journal ArticleDOI
An unusual adenosine cyclic 3',5'-phosphate-dependent protein kinase from Dictyostelium discoideum.
TL;DR: The differences in molecular and catalytic properties of this CAMP-dependent protein kinase with those from mammalian origin are discussed in relation with the possibility that the enzyme from Dictyostelium represents an early form of the molecule in the evolutionary process.
Journal ArticleDOI
Sensory transduction in eukaryotes
TL;DR: Compared sensory transduction in lower and higher eukaryotes general principles may be recognized that are found in all organisms and deviations that are present in specialised systems, which may help to understand the differences between cell types within one organism and the importance of a particular pathway.
Journal ArticleDOI
A cytosolic cyclic AMP-dependent protein kinase in Dictyostelium discoideum. I. Properties.
TL;DR: The CAMP-dependent protein kinase of the cellular slime mold, Dictyostelium discoideum, is developmentally regulated; there is an approximately 4-fold increase in activity during development, and the incorporation of 13H]leucine into the nzyme demonstrates that there is de novo synthesis of the CAMP
References
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Journal ArticleDOI
High resolution two-dimensional electrophoresis of proteins.
TL;DR: This technique provides a method for estimation of the number of proteins made by any biological system and can resolve proteins differing in a single charge and consequently can be used in the analysis of in vivo modifications resulting in a change in charge.
Journal ArticleDOI
Evidence for the existence of two types of cAMP binding sites in aggregating cells of Dictyostelium discoideum.
Anita A. Green,Peter C. Newell +1 more
TL;DR: Two classes of binding sites were demonstrated: Type 1, of low affinity and high capacity and Type 2, of high affinity but low capacity; the specificities of both sites are consistent with the specificity observed in vivo for the chemotactic response.
Journal ArticleDOI
The cyclic adenosine 3':5'-monophosphate receptor of Dictyostelium discoideum. Binding characteristics of aggregation-competent cells and variation of binding levels during the life cycle.
TL;DR: The discovery of two N-6-modified cAMP derivatives that compete with cAMP for binding sites opens the possibilities of using fluorescence quenching for assay of in vitro binding and of affinity labeling of binding sites.
Journal ArticleDOI
Characterization of a cyclic AMP-binding protein from bakers' yeast. Identification as a regulatory subunit of cyclic AMP-dependent protein kinase.
C S Hixson,E G Krebs +1 more
Journal ArticleDOI
Timers in developing systems.
TL;DR: Conditional methods for investigating the number and relationships of processes that are rate-limiting for the genesis of consecutive stages in a developmental sequence are proposed and an unexpected tentative scheme for timer relationships is obtained.
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A cAMP-dependent protein kinase is present in differentiating Dictyostelium discoideum cells.
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