Journal ArticleDOI
Immune stimulation by an antisense oligomer complementary to the rev gene of HIV-1.
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TLDR
Observations are consistent with the possibility that DNA sequences homologous to the rev gene participate in the regulation of mammalian lymphocyte activation, proliferation and maturation.About:
This article is published in Biochemical Pharmacology.The article was published on 1993-04-25. It has received 171 citations till now. The article focuses on the topics: Peripheral blood mononuclear cell & Spleen.read more
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CpG Motifs in Bacterial DNA and Their Immune Effects
TL;DR: Oligodeoxynucleotides containing CpG ODN enhance the development of acquired immune responses for prophylactic and therapeutic vaccination and protect against lethal challenge with a wide variety of pathogens.
Patent
Immunostimulatory nucleic acid molecules
TL;DR: In this paper, nucleic acids containing unmethylated CpG dinucleotides and therapeutic utilities based on their ability to stimulate an immune response and to redirect a Th2 response to a Th1 response in a subject are disclosed.
Journal ArticleDOI
CpG-DNA-specific activation of antigen-presenting cells requires stress kinase activity and is preceded by non-specific endocytosis and endosomal maturation.
Hans Häcker,Harald Mischak,Thomas Miethke,Susanne Liptay,Roland M. Schmid,Tim Sparwasser,Klaus Heeg,Grayson B. Lipford,Hermann Wagner +8 more
TL;DR: It is demonstrated that CpG‐DNA induces phosphorylation of Jun N‐terminal kinase kinase 1 (JNKK1/SEK/MKK4) and subsequent activation of the stress kinases JNK1/2 and p38 in murine macrophages and dendritic cells, and that cellular uptake via endocytosis and subsequent endosomal maturation is essential for signalling.
Patent
Use of nucleic acids containing unmethylated CpG dinucleotide as an adjuvant
TL;DR: In this article, a combination of immunostimulatory oligonucleotides having at least one unmethylated CpG dinucleotide (CpG ODN) and a non-nucleic acid adjuvant was used to induce cellular immunity in infants.
Journal ArticleDOI
Mechanism and Function of a Newly Identified CpG DNA Motif in Human Primary B Cells
TL;DR: The structure of a highly active human CpG motif is defined and its molecular mechanism of action in primary human B cells is characterized.
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Journal ArticleDOI
Abnormalities of B-cell activation and immunoregulation in patients with the acquired immunodeficiency syndrome.
TL;DR: The scope of immune dysfunction in the acquired immunodeficiency syndrome involves B cells as well as T cells, and it is concluded that the manifestations of B-cell hyperreactivity, such as hypergammaglobulinemia, seen in these patients are due to an in vivo polyclonal activation of B cells.
Journal ArticleDOI
Regulation of viral expression of human immunodeficiency virus in vitro by an antisense phosphorothioate oligodeoxynucleotide against rev (art/trs) in chronically infected cells.
Makoto Matsukura,Gerald Zon,Kazuo Shinozuka,Marjorie Robert-Guroff,Takashi Shimada,Cy A. Stein,Hiroaki Mitsuya,Flossie Wong-Staal,Jack S. Cohen,Samuel Broder +9 more
TL;DR: The altered HIV-1 mRNA profile induced by the antisense phosphorothioate oligomer suggests that the mechanism for the inhibition of viral expression is due to an interference with the regulatory gene, rev, by translation arrest.
Journal ArticleDOI
Molecular mechanisms of transmembrane signaling in B lymphocytes.
John C. Cambier,J T Ransom +1 more
TL;DR: A large and growing body of evidence attests to the complexity of the state of activation of B-lymphocyte activation by physiologic mediators, suggesting that each has a distinct receptor on the B-cell surface and also that respective receptors must be coupled to generation of distinct intracellular second messenger.
Journal Article
Phosphorothioate oligonucleotides are inhibitors of human DNA polymerases and RNase H: implications for antisense technology.
TL;DR: Results suggest that the non-sequence-specific inhibitory effect of S-oligos should be taken into consideration in designing antisense inhibitors.
Journal ArticleDOI
Structural analysis of the interaction between the human immunodeficiency virus Rev protein and the Rev response element
TL;DR: Increased reactivity to single-strand-specific probes of nucleotides in two helical domains indicates that Rev binding induces conformational changes in the RRE, and a Rev unit binding site is proposed that consists of a six-base-pair helical segment and three adjacent nucleotide.