Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Journal ArticleDOI
Efficient Enzymatic Preparation of Flavor Esters in Water
Igor Chiarelli Perdomo,Stefania Gianolio,Andrea Pinto,Diego Romano,Martina Letizia Contente,Martina Letizia Contente,Francesca Paradisi,Francesco Molinari +7 more
TL;DR: This enzymatic strategy represents an efficient alternative to the application of lipases in organic solvents and a significant improvement compared with already known methods in terms of reduced use of organicsolvents, paving the way to sustainable and efficient preparation of natural flavoring agents.
Journal ArticleDOI
Hydrolases on silica surfaces: Coverage-activity–molecular property relationships revealed
TL;DR: In this article, a set of recommendations to maintain high activity of immobilized enzymes is developed based on direct observation via AFM, and Molecule-level height and volume distribution analyses from high-resolution AFM images were applied to Candida antarctica Lipase B, subtilisin Carlsberg, and the Lipase from Thermomyces lanuginosus (TLL) deposited on model silica surfaces.
Journal ArticleDOI
Combined Enzyme and Transition-Metal Catalysis for Dynamic Kinetic Resolutions
Rocío Marcos,Belén Martín-Matute +1 more
TL;DR: In this paper, the preparation of optically pure alcohols, axially chiral allenes, and amine derivatives by using enzymes and transition-metal catalysts through dynamic kinetic resolution (DKR) is reviewed.
Journal ArticleDOI
Solid-phase peptide synthesis by ion-paired alpha-chymotrypsin in nonaqueous media.
TL;DR: In this article, the rate of coupling between N-alpha-benzyloxycarbonyl-L-phenylalanine methyl ester (Z-Phe-OMe) and leucine as a function of the reaction medium for both solid-phase and solution-phase reactions was examined.
Journal ArticleDOI
Acid stability of carbon paste enzyme electrodes.
TL;DR: This note reports on the unusual protection of several enzymes against harsh pH conditions provided by carbon paste electrodes, attributed to the "pH memory" of enzymes in the hydrophobic paste environment, to the barrier to hydronium ions provided by the pasting liquid and to decreased conformational mobility.
References
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Book
Principles of Biochemistry
TL;DR: The third edition, coming ten years after the first, emphasizes both the flowering of biochemical research and the prodigious effort by busy teachers and scientists to keep up to date this popular text and reference.
Book
Hydrogen Bonding in Biological Structures
TL;DR: In this article, the van der Waals Radii cut-off criterion is used to define the strong and weak hydrogen-bond configurations, as well as the relationship between two-center and three-center hydrogen bonds.
Book
Structure and Mechanism in Protein Science
TL;DR: The three-dimensional structure of proteins chemical catalysis the basic equations of enzyme kinetics measurement and magnitude of enzymatic rate constants the pH dependence of enzyme catalysis practical kinetics detection of intermediaries in reactions by kinetics stereochemistry of enzymes reactions active-site-directed and enzyme-activated irreversible inhibitors - affinity labels and suicide inhibitors conformational change, allosteric regulation, motors and work forces between molecules, and enzymesubstrate binding energies enzyme-substrate complementarity and the use of binding energy in catalysis specificity and editing mechanisms recombinant DNA technology case studies of enzyme
Journal ArticleDOI
Quantitative analyses of biochemical kinetic resolutions of enantiomers
BookDOI
Structure and Mechanism in Protein Science : a guide to enzyme catalysis and protein folding
TL;DR: The three-dimensional structure of proteins chemical catalysis, kinetics measurement and magnitude of enzymatic rate constants, and the use of binding energy in catalysis specificity and editing mechanisms are studied.