Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Highly stereoselective direct aldol reactions catalyzed by (S)-NOBIN-l-prolinamide
TL;DR: Acetone led to the aldol products in up to 93% ee, while cyclic ketones furnished the anti -aldols in moderate to high yield, excellent diastereoselectivity and high ee.
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An organic solvent-tolerant protease from Pseudomonas aeruginosa strain K: Nutritional factors affecting protease production
TL;DR: Significant protease production was observed with sodium nitrate as a sole nitrogen source however, ammonium nitrate completely inhibit it and addition of metal ions such as K , Mg 2+ and Ca 2+ maximized the enzyme production.
Journal ArticleDOI
On the Nature of Interactions between Ionic Liquids and Small Amino‐Acid‐Based Biomolecules
Alesia A. Tietze,Frank Bordusa,Ralf Giernoth,Diana Imhof,Thomas Lenzer,Astrid Maaß,Carmen Mrestani-Klaus,Ines Neundorf,Kawon Oum,Dirk Reith,Dirk Reith,Annegret Stark +11 more
TL;DR: This Review features a meta-analysis of the available data and findings to provide a basis for a scientifically profound understanding of the nature and mechanisms of interactions between ILs and structured or nonstructured peptides.
Journal ArticleDOI
Metabolism and motility in prebiotic structures.
TL;DR: Such motile agents would be capable of finding resources while escaping equilibrium and sustaining themselves through an internal metabolism, thus providing a working chemical model for a possible origin of life.
Journal ArticleDOI
Catalytic activity of laccases in aqueous solutions of ionic liquids
TL;DR: The ionic liquids, [bmim]Br and [BMim][N(CN)2] (where BMim = 1-butyl-3-methylimidazolium), stimulated laccase-catalysed oxidation of catechol when provided at concentrations between 10-20% and 50-60% (v/v) in water, respectively as discussed by the authors.
References
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