Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
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Journal ArticleDOI
Oxidation Catalysis by Enzymes in Microemulsions
TL;DR: Nanodispersed biocatalytic media are perfect hosts for liquid oxidation reactions catalyzed by many enzymes such as heme peroxidases, phenoloxidase, cholesterol oxidase, and dehydrogenases and the system’s composition and structural properties are important for better understanding of nanodispersion-biocatalyst interactions.
Journal ArticleDOI
Diverse effects of aqueous polar co-solvents on Candida antarctica lipase B.
Marco Mangiagalli,Henrique F. Carvalho,Antonino Natalello,Valerio Ferrario,Matteo Luigi Pennati,Alberto Barbiroli,Marina Lotti,Juergen Pleiss,Stefania Brocca +8 more
TL;DR: Methanol and acetone were found to act as competitive inhibitors of rCALB and to promote its aggregation, whereas DMSO is a non-essential activator of the recombinant Candida antarctica lipase B.
Journal ArticleDOI
A facile whole-cell biocatalytic approach to regioselective synthesis of monoacylated 1-β-D-arabinofuranosylcytosine: influence of organic solvents.
TL;DR: The lyophilized Pseudomonas fluorescens cell was an efficient alternative catalyst to enzymes for highly regioselective acylation of a polar nucleoside, 1-β-D-arabinofuranosylcytosine (ara-C), which could also have environmental and cost advantages.
Journal ArticleDOI
Resolution of racemic ketoprofen in organic solvents by lipase from Burkholderia cepacia G63
TL;DR: A lipase from the Burkholderia cepacia strain G63 immobilized on resin was used for the resolution of ketoprofen and exhibited a better enantioselectivity than some commercial lipases, such as Novozym 435, Lipozyme RM IM and Lipo enzymeTL IM.
Journal ArticleDOI
Efficient synthesis of novel 1α-amino and 3β-amino analogues of 1α,25-dihydroxyvitamin D3
TL;DR: In this paper, the A-ring fragments were constructed by selective enzymatic hydrolysis of a diester intermediate and introduction of the amino group under Mitsunobu conditions.
References
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