Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Enzymatic biofuel cells based on protective hydrophobic carbon paste electrodes: towards epidermal bioenergy harvesting in the acidic sweat environment.
Juliane R. Sempionatto,Paulo A. Raymundo-Pereira,Paulo A. Raymundo-Pereira,Nathália F. B. Azeredo,Nathália F. B. Azeredo,Andre N. De Loyola e Silva,Lúcio Angnes,Joseph Wang +7 more
TL;DR: It is demonstrated that the encapsulation of lactate-oxidase (LOx) within a hydrophobic protective carbon-paste anode imparts unusually high stability during dynamically changing pH fluctuations and allows the BFC to continue harvesting the lactate bioenergy even after long exposures to acidic conditions.
Journal ArticleDOI
Hemoglobin‐Catalyzed Synthesis of Indolizines Under Mild Conditions
Fengxi Li,Xuyong Tang,Yaning Xu,Chunyu Wang,Liu Zhang,Jiaxin Zhang,Jiaxu Liu,Zhengqiang Li,Lei Wang +8 more
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Reactive Separations for In Situ Product Removal of Enzymatic Reactions: A Review
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Four Challenges for Better Biocatalysts
TL;DR: This review summarises four of the key challenges in understanding the fundamental biochemistry of enzymes: the need to understand protein folding, the need for a qualitative understanding of the hydrophobic effect, theneed to understand and quantify the effects of organic solvents on biomolecules and theneed for a deep understanding of enzymatic catalysis.
Journal ArticleDOI
Synthesis of designer triglycerides by enzymatic acidolysis.
TL;DR: Synthesis of modified fats by enzymatic acidolysis of fully hydrogenated soybean oil with caprylic acid and significant change in the physico-chemical properties of newly formed product as observed using DSC and XRD analysis.
References
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TL;DR: The third edition, coming ten years after the first, emphasizes both the flowering of biochemical research and the prodigious effort by busy teachers and scientists to keep up to date this popular text and reference.
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Quantitative analyses of biochemical kinetic resolutions of enantiomers
BookDOI
Structure and Mechanism in Protein Science : a guide to enzyme catalysis and protein folding
TL;DR: The three-dimensional structure of proteins chemical catalysis, kinetics measurement and magnitude of enzymatic rate constants, and the use of binding energy in catalysis specificity and editing mechanisms are studied.