Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Journal ArticleDOI
Lipase-catalyzed aza-Michael reaction on acrylate derivatives.
Peter Steunenberg,Maarten Sijm,Han Zuilhof,Han Zuilhof,Johan P. M. Sanders,Elinor L. Scott,Maurice C. R. Franssen +6 more
TL;DR: Under the optimal conditions it was found that Pseudomonas stutzeri lipase and Chromobacterium viscosum lipase showed the highest selectivity for the aza-Michael addition to substituted alkyl acrylates.
Patent
Process for producing glutamate derivatives
Masakazu Sugiyama,Kunihiko Watanabe,Nao Funakoshi,Yusuke Amino,Shigeru Kawahara,Tadashi Takemoto +5 more
TL;DR: In this article, a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted α-keto acid of formula (1) into a glutamamic acid derivative of formula(2) in the presence of an enzyme catalyzing conversion of the same.
Journal ArticleDOI
Pickering stabilized peptide gel particles as tunable microenvironments for biocatalysis.
TL;DR: The combination of a tunable chemical environment in gel phase and Pickering stabilization described here is expected to prove useful for areas where proteins are to be exploited in technological contexts such as biocatalysis and also in other areas where protein performance and activity are important, such as biosensors and bioinspired solar fuel devices.
Journal ArticleDOI
The solvent influence on the positional selectivity of Novozym 435 during 1,3-diolein synthesis by esterification.
Zhang-Qun Duan,Wei Du,Dehua Liu +2 more
TL;DR: It was revealed that relatively hydrophilic solvent such as t-butanol was an ideal solvent for Novozym 435 catalyzed 1,3-diolein synthesis through esterification of oleic acid with glycerol.
Journal ArticleDOI
Purification and biological characterization of a halophilic thermostable protease from Haloferax lucentensis VKMM 007
TL;DR: The proteolytic activity remained stable or only marginally inhibited in the presence of various polar and non-polar solvents, surfactants and reducing agents thus emphasizing the biotechnological potential of this novel halophilic protease.
References
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