Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Journal ArticleDOI
Efficient synthesis of phosphatidylserine in 2-methyltetrahydrofuran.
Zhang-Qun Duan,Fei Hu +1 more
TL;DR: Results indicated that as high as 90% yield of phosphatidylserine could be achieved after 12 h combined with no byproduct (phosphatidic acid) forming, and this work is a proof-of-concept opening the use of 2-methyltetrahydrofuran in biosynthesis as well.
Journal ArticleDOI
Highly sensitive active-site titration of lipase in microscale culture media using fluorescent organophosphorus ester
TL;DR: The present active-site titration method is widely applicable to the biocatalytic engineering of lipases such as directed evolution, site-directed mutagenesis, chemical modification and immobilization.
Journal ArticleDOI
Enzymatic catalysis in gel-stabilized two-phase systems: improvement of the solvent phase
Bastien Doumèche,Matthias Heinemann,Jochen Büchs,Winfried Hartmeier,Marion B. Ansorge-Schumacher +4 more
TL;DR: In this paper, an improvement of the solvent phase with special regard to the avoidance of gel agglomeration in batch as well as fluidized-bed reactors, and resulting effects on the catalyzed reaction was provided.
Journal ArticleDOI
Comparison of catalysis by haloalkane dehalogenases in aqueous solutions of deep eutectic and organic solvents
TL;DR: In this article, the performance of three haloalkane dehalogenases, DbjA, DhaA and LinB, in aqueous solutions of the deep eutectic solvent ethaline, its components (ethylene glycol and choline chloride), and two organic solvents (methanol and acetone) was systematically and critically compared.
Journal ArticleDOI
Enzyme dynamics: Looking beyond a single structure.
TL;DR: A review of recent developments, several challenges as well as opportunities related to enzyme dynamics and its role in catalysis are discussed.
References
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Book
Principles of Biochemistry
TL;DR: The third edition, coming ten years after the first, emphasizes both the flowering of biochemical research and the prodigious effort by busy teachers and scientists to keep up to date this popular text and reference.
Book
Hydrogen Bonding in Biological Structures
TL;DR: In this article, the van der Waals Radii cut-off criterion is used to define the strong and weak hydrogen-bond configurations, as well as the relationship between two-center and three-center hydrogen bonds.
Book
Structure and Mechanism in Protein Science
TL;DR: The three-dimensional structure of proteins chemical catalysis the basic equations of enzyme kinetics measurement and magnitude of enzymatic rate constants the pH dependence of enzyme catalysis practical kinetics detection of intermediaries in reactions by kinetics stereochemistry of enzymes reactions active-site-directed and enzyme-activated irreversible inhibitors - affinity labels and suicide inhibitors conformational change, allosteric regulation, motors and work forces between molecules, and enzymesubstrate binding energies enzyme-substrate complementarity and the use of binding energy in catalysis specificity and editing mechanisms recombinant DNA technology case studies of enzyme
Journal ArticleDOI
Quantitative analyses of biochemical kinetic resolutions of enantiomers
BookDOI
Structure and Mechanism in Protein Science : a guide to enzyme catalysis and protein folding
TL;DR: The three-dimensional structure of proteins chemical catalysis, kinetics measurement and magnitude of enzymatic rate constants, and the use of binding energy in catalysis specificity and editing mechanisms are studied.