Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Journal ArticleDOI
A novel control of enzymatic enantioselectivity through the racemic temperature influenced by reaction media.
TL;DR: An effective approach to the control of the enzymatic enantioselectivity and the prediction of the increasing tendency was developed based on the T(r) influenced by reaction media.
Journal ArticleDOI
Circular dichroism and UV resonance Raman study of the impact of alcohols on the Gibbs free energy landscape of an α-helical peptide
Kan Xiong,Sanford A. Asher +1 more
TL;DR: This work used CD and UV resonance Raman spectroscopy to study the impact of alcohols on the conformational equilibria and relative Gibbs free energy landscapes along the Ramachandran Psi-coordinate of a mainly poly-Ala peptide with an AAAAA(AAARA)(3)A sequence and found that while TFE induces more alpha-helices, it favors multiple, shorter helix segments.
Journal ArticleDOI
Hemoglobin: A New Biocatalyst for the Synthesis of 2‐substituted Benzoxazoles via Oxidative Cyclization
TL;DR: Efficient and mild synthesis of a series of 2‐substituted benzoxazoles via oxidative cyclization catalyzed by hemoglobins reported here for the first time.
Journal ArticleDOI
Synergy between chemo- and bio-catalysts in multi-step transformations
TL;DR: In this article, the authors combined a lipase catalyzed amidation with palladium catalyzed coupling reactions, simultaneously carried out on the same molecule, and found that the bio-and chemo-catalyzed processes show synergistic behavior, highlighting the complexity of multi-catalyst systems.
Journal ArticleDOI
Modeling and optimization of phenylalanine ammonia lyase stabilization in recombinant Escherichia coli for the continuous synthesis of l-phenylalanine on the statistical-based experimental designs.
TL;DR: PAL stability was significantly improved during successive bioconversion and may be helpful for l-Phe production on an industrial scale.
References
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Quantitative analyses of biochemical kinetic resolutions of enantiomers
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