Journal ArticleDOI
Improving enzymes by using them in organic solvents
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TLDR
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media, and they have found numerous potential applications, some of which are already commercialized.Abstract:
The technological utility of enzymes can be enhanced greatly by using them in organic solvents rather than their natural aqueous reaction media. Studies over the past 15 years have revealed not only that this change in solvent is feasible, but also that in such seemingly hostile environments enzymes can catalyse reactions impossible in water, become more stable, and exhibit new behaviour such as 'molecular memory'. Of particular importance has been the discovery that enzymatic selectivity, including substrate, stereo-, regio- and chemoselectivity, can be markedly affected, and sometimes even inverted, by the solvent. Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized.read more
Citations
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Journal ArticleDOI
Repurposing Biocatalysts to Control Radical Polymerizations
Kyle J. Rodriguez,Bernadetta Gajewska,Jonas Pollard,Michela M. Pellizzoni,Csaba Fodor,Nico Bruns +5 more
TL;DR: The scope of biocatalytic atom transfer radical polymerizations (bioATRP), enzyme-initiated reversible addition–fragmentation chain transfer radical Polymerization is critically reviewed, and the potential of these reactions for the environmentally friendly synthesis of precision polymers, for the preparation of functional nanostructures, and so on are critically reviewed.
Journal ArticleDOI
Biological chemistry: Dehydrated but unharmed.
TL;DR: A study in the vacuum chamber of a mass spectrometer reveals that the weakest interactions of protein complexes are thought to be lost when such assemblies are removed from their natural, watery environments.
Journal ArticleDOI
Enzyme Hydration Determines Resistance in Organic Cosolvents
Haiyang Cui,Lingling Zhang,Lobna Eltoukhy,Qianjia Jiang,Seval Kübra Korkunç,Karl-Erich Jaeger,Karl-Erich Jaeger,Ulrich Schwaneberg,Ulrich Schwaneberg,Mehdi D. Davari +9 more
TL;DR: In this paper, the authors describe the use of biocatalysis in organic solvents (OSs) for organic synthesis and for the production of pharmaceuticals, flavors, and fragrances.
Journal ArticleDOI
TBADH activity in water-miscible organic solvents: correlations between enzyme performance, enantioselectivity and protein structure through spectroscopic studies.
TL;DR: It is shown that the tetrameric form of TBADH is not critical for catalytic performance, and changes in enzyme activity as a function of organic solvent were correlated to structural alterations of TBadH with a series of spectroscopic studies.
Journal ArticleDOI
Engineered Surface-Immobilized Enzyme that Retains High Levels of Catalytic Activity in Air
Somayesadat Badieyan,Qiuming Wang,Xingquan Zou,Yaoxin Li,Maggie Herron,Nicholas L. Abbott,Zhan Chen,E. Neil G. Marsh +7 more
TL;DR: Poly(sorbitol methacrylate) appears to enhance activity by replacing protein-water interactions, thereby preserving the protein structure in the immobilized enzyme.
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