Book ChapterDOI
In Vitro Biosynthesis of Polyphenols in the Presence of Elicitors and Upregulation of Genes of the Phenylpropanoid Pathway in Plantago ovata
Debangana Kundu,Pratik Talukder,Sarmistha Sen Raychaudhuri +2 more
- Vol. 60, pp 299-344
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TLDR
In this paper, a review of the properties of polyphenols in callus culture of Plantago ovata is presented, which is an important medicinal plant and a rich source of secondary metabolites including polyphenolic compounds.Abstract:
Plantago ovata is an important medicinal plant and a rich source of secondary metabolites including polyphenolic compounds. In vitro callus culture of this plant opens up the possibility of photochemical prospecting with enhanced production of some polyphenols using elicitors. Utilizing the callus is advantageous as the whole plant need not be sacrificed. Subculturing results in an increase of callus biomass along with the most prized phytochemicals. The reservoir of polyphenolic compounds has been studied by various authors from time to time to extract polyphenols in general and some others that are unique to Plantago by HPLC analysis, such as trans-cinnamic acid, gallic acid, rutin, quercetin, catechin, luteolin-7-O-β- d glucoside, coumaric acid, vanillic acid, chlorogenic acid, trans-ferulic acid, and caffeic acid. Organic plant additives, such as casein hydrolysate, coconut water, and even plant growth regulators in different concentrations and combinations, have resulted in enhancing polyphenol and flavonoid biosynthesis during in vitro callus culture of this plant. Polyphenols, being natural products, result in higher antioxidant activity in subcultured calli. Epidemiological studies have shown that a human diet rich in polyphenols gives protection against several metabolic disorders, such as diabetes, cancer, and cardiovascular diseases. Polyphenols are intermediate products of the phenylpropanoid pathway and their production is regulated by differential expression of the genes of this pathway, such as phenylalanine ammonia lyase (PAL), chalcone synthase (CHS), and dihydroflavonol-4-reductase (DFR). Another important gene is polyphenol oxidase (PPO), which causes enzymatic browning by oxidation of phenolic compounds to O-quinone and polymerizes polyphenolic compounds. It is regarded as an antioxidative defense enzyme of plants. Critical quantitation of the organic chemicals that enhance the production of polyphenols will be dealt with in this review chapter in relation to PAL, CHS, DFR, and PPO. This will clarify biochemical regulation by using molecular biological techniques like reverse transcription and real-time PCR. Bioinformatic details on the structure prediction of these genes will also throw light on the upregulation of gene expression in relation to increased polyphenol biosynthesis.read more
Citations
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Journal ArticleDOI
Development of the Polyphenol Compounds from Plant Raw Materials by the Cell Micropropagation IN VITRO Method
TL;DR: In this article , the authors used Kundu phytohormones to regulate secondary metabolites synthesis in plant cells, and they used Murashige Skoog and Anderson agar media to find the optimal nutrient medium in order to obtain the maximum increase in polyphenolic compounds.
References
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Tomato phenylalanine ammonia-lyase gene family, highly redundant but strongly underutilized.
TL;DR: Southern blot and dot blot analyses in tomato indicate a surprisingly large family of related sequences with ∼26 copies in the diploid genome, some easily distinguished by restriction enzyme digestion, suggesting aggressive competition between PAL gene duplication and copy inactivation during PAL gene evolution.
Journal ArticleDOI
Stress responses in alfalfa ( Medicago sativa L.). 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family
TL;DR: The induction kinetics of CHS2 transcripts were more rapid and/or transient than those of other members of the CHS family in CuCl2-treated roots and Phoma-infected leaves.
Journal ArticleDOI
Duplication and functional divergence in the chalcone synthase gene family of Asteraceae: evolution with substrate change and catalytic simplification.
Yrjö Helariutta,Mika Kotilainen,Paula Elomaa,Nisse Kalkkinen,Kåre Bremer,Teemu H. Teeri,Victor A. Albert +6 more
TL;DR: This study confirms a gene duplication-based model that explains how various related functions have arisen from CHS during plant evolution and isolated an unusual CHS-like gene, GCHS2, from Gerbera hybrida (Asteraceae.
Journal ArticleDOI
Alkaloid production in cultured roots of three species of Duboisia
Tsuyoshi Endo,Yasuyuki Yamada +1 more
TL;DR: Cultured roots were obtained from calluses of Duboisia leichhardtii, D. myoporoides and D. hopwoodii and produced both tropane and pyridine-type alkaloids.
Journal ArticleDOI
Tyrosinases from crustaceans form hexamers.
Elmar Jaenicke,Heinz Decker +1 more
TL;DR: The hexameric nature of arthropod tyrosinases suggests that these proteins were the ideal predecessors from which to develop the oxygen-carrier protein haemocyanin, with its allosteric and co-operative properties, later on.