Journal ArticleDOI
Incorporation of cis-parinaric acid, a fluorescent fatty acid, into synaptosomal phospholipids by an acyl-CoA acyltransferase
TLDR
It is proposed that cis-parinaric acid, like fatty acids normally found in brain, is incorporated into membrane phospholipids by an acyl-CoA acyltransferase, and the presence of this enzyme in nervous tissue may make it possible to easily introduce fluorescent fatty acid probes into membrane phosphate membranes and to thereby facilitate study of membrane-mediated processes.About:
This article is published in Biochimica et Biophysica Acta.The article was published on 1983-12-07. It has received 10 citations till now. The article focuses on the topics: Parinaric Acid & Fatty acid.read more
Citations
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Journal ArticleDOI
Interleukin-1 rapidly stimulates lysophosphatidate acyltransferase and phosphatidate phosphohydrolase activities in human mesangial cells.
TL;DR: It is concluded that PA is a significant signaling intermediary for IL-1 via activation of lyso-PA AT and a G-protein, which activates phosphatidate phosphohydrolase, suggesting a novel mechanism whereby a low intensity signal may be translated into cellular activation.
Journal ArticleDOI
Valproic acid selectively inhibits conversion of arachidonic acid to arachidonoyl-CoA by brain microsomal long-chain fatty acyl-CoA synthetases: relevance to bipolar disorder
Richard P. Bazinet,Margaret T. Weis,Stanley I. Rapoport,Thad A. Rosenberger,Thad A. Rosenberger +4 more
TL;DR: The study supports the hypothesis that valproic acid acts in bipolar disorder by reducing the brain arachidonic acid cascade, by inhibiting arachidonoyl–CoA formation.
Journal ArticleDOI
An esterification protocol for cis-parinaric acid-determined lipid peroxidation in immune cells1,2
TL;DR: Esterification of cPnA into membrane phospholipids can sensitively detect changes in lipid peroxidation induced by alteration of membrane PUFA and/or vitamin E content.
Book ChapterDOI
Fluorescence in Membranes
TL;DR: Recent advances in fluorescence microscopy techniques have allowed scientists to visualize microscopic perturbations of individual vesicles and have allowed the study of membrane dynamics at higher spatiotemporal resolution than before.
Journal ArticleDOI
Rapid activation of phosphatidate phosphohydrolase in mesangial cells by lipid A.
Stuart L. Bursten,Ward E. Harris +1 more
TL;DR: It is proposed that specific pools of PA and PA-derived DAG are key elements in rapid signaling in mesangial cells and are independent of the PI cycle and phospholipase C.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Journal ArticleDOI
The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes
TL;DR: The effects of the ionic strength and pH of the hemolyzing solution on the hemoglobin content of human erythrocyte ghosts were studied in phosphate buffers and suggest an electrophysical interaction of hemoglobin with membrane constituents.
BookDOI
The Enzymes of Biological Membranes
TL;DR: Electron Microscopy of Biological Membranes: A Completely Membrane Associated Cytoskeleton and Lipid Polymorphism: Technical Aspects and "Isotropic" Lipid Structures and Lipids.
Journal ArticleDOI
Metabolism of Glycerolipids: II. THE ENZYMATIC ACYLATION OF LYSOLECITHIN
TL;DR: The properties of the microsomal system which catalyzes the acylation of lysolecithin to form leCithin are described, which requires ATP and CoA, and involves the formation of an acyl-CoA derivative.