Journal ArticleDOI
Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh.
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TLDR
The system of non-germinating mutants offers a resolving power unique in higher plants, so that self-detecting rare events like induced revertants or intragenic recombinants can be efficiently screened for.Abstract:
In Arabidopsis thaliana 37 independent irradiation or EMS induced mutants were isolated which have an absolute or almost absolute gibberellin (GA) requirement for germination and successive elongation growth. These are called 'non-germinating GA-dwarfs', since without further addition of GA they develop into typical GA-dwarfs, being dark green, stunted and sterile. However, with repeated GA-treatment they develop into fertile plants with a completely wild type phenotype, or nearly so. In addition, 19 independently induced 'germinating GA-dwarfs' were obtained, i.e. mutants which do germinate without GA but develop into typical GA-dwarfs. With repeated GA-treatment these too grow to become completely wild type phenotypes, or nearly so. 'Germinating dwarfs' have been found by previous authors in a number of other plant species. The 'non-germinating dwarfs' form a new class of mutants. The system of non-germinating mutants offers a resolving power unique in higher plants, so that self-detecting rare events like induced revertants or intragenic recombinants can be efficiently screened for.The 56 GA-sensitive mutants represent mutations at 5 loci, located on three of five Arabidopsis chromosomes. At three of the five loci both mutant classes were represented in similar frequency ratio's, whilst at the other two loci only germinating dwarfs were found.read more
Citations
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Journal ArticleDOI
Gibberellin Metabolism and its Regulation
TL;DR: Current understanding of the GA biosynthesis and deactivation pathways in plants and fungi is summarized, and how GA concentrations in plant tissues are regulated during development and in response to environmental stimuli is discussed.
Journal ArticleDOI
The CONSTANS gene of arabidopsis promotes flowering and encodes a protein showing similarities to zinc finger transcription factors
TL;DR: Double mutants were constructed containing co and mutations affecting gibberellic acid responses, meristem identity, or phytochrome function, and their phenotypes suggested a model for the role of CO in promoting flowering.
Journal ArticleDOI
The Arabidopsis GAI gene defines a signaling pathway that negatively regulates gibberellin responses
Jinrong Peng,Pierre Carol,Donald Ernest Richards,Kathryn E. King,Rachel J. Cowling,George Murphy,Nicholas P. Harberd +6 more
TL;DR: The molecular cloning ofGAI and a closely related gene GRS is reported, indicating the involvement of GAI, SPY, and GAR2 in a signaling pathway that regulates GA responses negatively and suggests that GA modulates plant growth through derepression rather than through simple stimulation.
Journal ArticleDOI
Seed Dormancy and Germination
TL;DR: In this article, the role of the plant hormones abscisic acid and gibberellin in the regulation of seed dormancy and germination has been investigated in Arabidopsis thaliana.
Journal ArticleDOI
Activation tagging in Arabidopsis.
Detlef Weigel,Ji Hoon Ahn,Miguel A. Blázquez,Justin O. Borevitz,Sioux K. Christensen,Christian Fankhauser,Cristina Ferrándiz,Igor Kardailsky,Igor Kardailsky,Elizabeth J. Malancharuvil,Michael M. Neff,Michael M. Neff,Jasmine Thuy Nguyen,Shusei Sato,Zhi-Yong Wang,Yiji Xia,Richard A. Dixon,Maria J. Harrison,Christopher J. Lamb,Christopher J. Lamb,Martin F. Yanofsky,Joanne Chory,Joanne Chory +22 more
TL;DR: Activation tagging using T-DNA vectors that contain multimerized transcriptional enhancers from the cauliflower mosaic virus (CaMV) 35S gene has been applied to Arabidopsis plants, and it is shown that overexpressed genes are almost always found immediately adjacent to the inserted CaMV 35S enhancers.
References
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Journal ArticleDOI
The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana (L.) heynh
TL;DR: By selecting for germinating seeds in the progeny of mutagen-treated non-germinating gibberellin responsive dwarf mutants of the ga−1 locus in Arabidopsis thaliana, Germinating lines (revertants) could be isolated.
Journal ArticleDOI
Isolation and characterization of chlorate-resistant mutants of Arabidopsis thaliana
TL;DR: Chlorate-resistant mutants of Arabidopsis thaliana were isolated in order to find nitrate reductase-less mutants and two mutants of the second type are described, which probably have a much lowered uptake of chlorate.
Journal ArticleDOI
Abnormal stomatal behavior and root resistance, and hormonal imbalance in three wilty mutants of tomato.
TL;DR: It has been suggested that the first hormonal change in the mutants is that of abscisic acid, and from it proceed the other changes.
Single locus heterosis
TL;DR: Experimental evidence is reported in favor of superdominance in over-all vigor in Arabidopsis thaliana in connection with two x-ray induced mutants.