Journal ArticleDOI
Infection patterns of o'nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae.
Aaron C. Brault,Brian D. Foy,Kevin M. Myles,Cindy L. H. Kelly,Stephen Higgs,Scott C. Weaver,Ken E. Olson,Barry R. Miller,Ann M. Powers,Ann M. Powers +9 more
TLDR
A series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes, that will be a valuable asset in parasite–mosquito interaction and interference research and to serve as tools for antimalaria studies.Abstract:
Arthropod-borne alphaviruses transmitted by mosquitoes almost exclusively use culicines; however, the alphavirus o'nyong-nyong (ONNV) has the unusual characteristic of being transmitted primarily by anopheline mosquitoes This unusual attribute makes ONNV a valuable tool in the characterization of mosquito determinants of infection as well as a useful expression system in Anopheles species We developed a series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes The backbone genome is a full-length infectious cDNA clone of ONNV from which wild-type virus can be rescued Additional constructs are variants of the primary clone and contain the complete genome plus a duplicated subgenomic promoter element with a multiple cloning site for insertion of heterologous genes We inserted a green fluorescent protein (GFP) gene downstream of this promoter and used it to characterize infection and dissemination patterns of ONNV within An gambiae mosquitoes These experiments allowed us to identify atypical sites of initial infection and dissemination patterns in this mosquito species not frequently observed in comparable culicine infections The utility of these ONNVs for studies in anopheline mosquitoes includes the potential for identification of vector infection determinants and to serve as tools for antimalaria studies Viruses that can express a heterologous gene in a vector and rapidly and efficiently infect numerous tissues in An gambiae mosquitoes will be a valuable asset in parasite-mosquito interaction and interference researchread more
Citations
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Journal ArticleDOI
Anopheles gambiae heat shock protein cognate 70B impedes o'nyong-nyong virus replication
Cheolho Sim,Cheolho Sim,Young S. Hong,Konstantin A. Tsetsarkin,Dana L. Vanlandingham,Stephen Higgs,Frank H. Collins +6 more
TL;DR: Results indicate that HSC70B plays important roles in homeostasis and suppression of ONNV replication in the vector, An.
Book ChapterDOI
The Anopheles Mosquito Microbiota and Their Impact on Pathogen Transmission
TL;DR: An ecosystem is composed of a biological community and its physical environment, which contains and interacts with many microor‐ ganisms, e.g. a single human gut contains 1013-1014 bacteria belonging to hundreds of species.
Journal ArticleDOI
Fighting Arbovirus Transmission: Natural and Engineered Control of Vector Competence in Aedes Mosquitoes
Joy Kean,Stephanie M. Rainey,Melanie McFarlane,Claire L. Donald,Esther Schnettler,Alain Kohl,Emilie Pondeville +6 more
TL;DR: This review will focus on the development and application of biological approaches, both natural or engineered, to limit mosquito vector competence for arboviruses, and the use of endosymbiotic bacteria such as Wolbachia as well as entomopathogenic fungi, which are proposed strategies to control vector competence.
Journal ArticleDOI
Characterization of Novel Splice Variants of Zinc Finger Antiviral Protein (ZAP).
Melody M. H. Li,Eduardo G. Aguilar,Eleftherios Michailidis,Jonathan Pabon,Paul Park,Xianfang Wu,Ype P. de Jong,Ype P. de Jong,William M. Schneider,Henrik Molina,Charles M. Rice,Margaret R. MacDonald +11 more
TL;DR: This study characterized the antiviral, translational inhibition, and IFN activation activities of individual ZAP isoforms by taking a gene-knockout-and-reconstitution approach and shows that the full spectrum of ZAP activities can change depending on the virus target and the relative levels of basal expression and induction by IFN or infection.
Journal ArticleDOI
O'nyong nyong virus molecular determinants of unique vector specificity reside in non-structural protein 3.
Kali D. Saxton-Shaw,Jeremy P. Ledermann,Erin M. Borland,Janae L. Stovall,Eric C. Mossel,Eric C. Mossel,Amber J. Singh,Jeffrey Wilusz,Ann M. Powers +8 more
TL;DR: It is shown that a non-structural genome element involved in viral replication is a major element involvedIn conclusion, ONNV is the only alphavirus transmitted by anopheline mosquitoes with a unique vector specificity.
References
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Journal ArticleDOI
Re-emergence of Chikungunya and O'nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships.
TL;DR: Phylogenetic trees corroborated historical evidence that CHIK virus originated in Africa and subsequently was introduced into Asia and revealed that ONN virus is indeed distinct from CHIK viruses, and these viruses probably diverged thousands of years ago.
Journal ArticleDOI
Stable germline transformation of the malaria mosquito Anopheles stephensi
Flaminia Catteruccia,Tony Nolan,Thanasis G. Loukeris,Claudia Blass,Charalambos Savakis,Fotis C. Kafatos,Andrea Crisanti +6 more
TL;DR: A transposon, based on the Minos element and bearing exogenous DNA, can integrate efficiently and stably into the germ line of the human malaria vector Anopheles stephensi, through a transposase-mediated process.
Journal ArticleDOI
Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element.
Genelle L. Grossman,Cristina S. Rafferty,John R. Clayton,T. K. Stevens,O. Mukabayire,Mark Q. Benedict +5 more
TL;DR: An improvement in the injection technique at the end of the studies resulted in increased G0 hatching, transient expression and EGFP‐expression rates among G1 progeny, and genetic cross data suggest that the insertion site of the element either resulted in, or is tightly linked to, a recessive lethal.
Journal ArticleDOI
Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti.
TL;DR: The comparative susceptibility of 13 geographic strains of Aedes aegypti to oral infection with dengue viruses was studied by feeding the mosquitoes on a virus-erythrocyte-sugar suspension, suggesting that the factors controlling susceptibility were the same for all types.