Journal ArticleDOI
Infection patterns of o'nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae.
Aaron C. Brault,Brian D. Foy,Kevin M. Myles,Cindy L. H. Kelly,Stephen Higgs,Scott C. Weaver,Ken E. Olson,Barry R. Miller,Ann M. Powers,Ann M. Powers +9 more
TLDR
A series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes, that will be a valuable asset in parasite–mosquito interaction and interference research and to serve as tools for antimalaria studies.Abstract:
Arthropod-borne alphaviruses transmitted by mosquitoes almost exclusively use culicines; however, the alphavirus o'nyong-nyong (ONNV) has the unusual characteristic of being transmitted primarily by anopheline mosquitoes This unusual attribute makes ONNV a valuable tool in the characterization of mosquito determinants of infection as well as a useful expression system in Anopheles species We developed a series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes The backbone genome is a full-length infectious cDNA clone of ONNV from which wild-type virus can be rescued Additional constructs are variants of the primary clone and contain the complete genome plus a duplicated subgenomic promoter element with a multiple cloning site for insertion of heterologous genes We inserted a green fluorescent protein (GFP) gene downstream of this promoter and used it to characterize infection and dissemination patterns of ONNV within An gambiae mosquitoes These experiments allowed us to identify atypical sites of initial infection and dissemination patterns in this mosquito species not frequently observed in comparable culicine infections The utility of these ONNVs for studies in anopheline mosquitoes includes the potential for identification of vector infection determinants and to serve as tools for antimalaria studies Viruses that can express a heterologous gene in a vector and rapidly and efficiently infect numerous tissues in An gambiae mosquitoes will be a valuable asset in parasite-mosquito interaction and interference researchread more
Citations
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Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity
John W. Schoggins,Donna A. MacDuff,Naoko Imanaka,Maria D. Gainey,Bimmi Shrestha,Jennifer L. Eitson,Katrina B. Mar,R. Blake Richardson,Alexander V. Ratushny,Vladimir Litvak,Rea Dabelic,Balaji Manicassamy,John D. Aitchison,Alan Aderem,Richard M. Elliott,Adolfo García-Sastre,Vincent R. Racaniello,Eric J. Snijder,Wayne M. Yokoyama,Michael S. Diamond,Herbert W. Virgin,Charles M. Rice +21 more
TL;DR: Comparative analysis reveals that the screened ISGs target positive-sense single-stranded RNA viruses more effectively than negative-sensesingle-strander RNA viruses.
Journal ArticleDOI
Tissue Barriers to Arbovirus Infection in Mosquitoes.
TL;DR: The nature of the tissue barriers that arboviruses are confronted with in a mosquito vector and how they might surmount these barriers are explained.
Journal ArticleDOI
Aedes aegypti uses RNA interference in defense against Sindbis virus infection
Corey L. Campbell,Kimberly M. Keene,Douglas E Brackney,Ken E. Olson,Carol D. Blair,Jeffrey Wilusz,Brian D. Foy +6 more
TL;DR: It is shown that silencing RNAi components in Ae.
Journal ArticleDOI
Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
TL;DR: It is suggested that an exogenous siRNA pathway is essential to the survival of mosquitoes infected with alphaviruses and, thus, the maintenance of these viruses in nature.
Journal ArticleDOI
Infectious clones of Chikungunya virus (La Réunion isolate) for vector competence studies.
Konstantin A. Tsetsarkin,Stephen Higgs,Charles E. McGee,Xavier de Lamballerie,Rémi N. Charrel,Dana L. Vanlandingham +5 more
TL;DR: Comparison of the growth kinetics and infection rates of the viral isolate CHIKV strain LR2006 OPY1 (CHIKV-LR) and a full-length infectious clone indicate that the infectious clone has retained the viral phenotypes of the original isolate.
References
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Midgut and salivary gland barriers to La Crosse virus dissemination in mosquitoes of the Aedes triseriatus group.
TL;DR: In this paper, vector competence for La Crosse virus (LACV) was compared for four species in the Aedes triseriatus group: Ae.triseriatus (Say), Ae.hendersoni Cockerel], Ae.zoosophus Dyar and Knab and Ae.brelandi Zavortink (Diptera: Culicidae).
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Development of a Sindbis virus expression system that efficiently expresses green fluorescent protein in midguts of Aedes aegypti following per os infection.
TL;DR: The MRE3′2 J dsSIN expression system should be an important tool for efficient gene expression in Ae.
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Deletions in the Putative Cell Receptor-Binding Domain of Sindbis Virus Strain MRE16 E2 Glycoprotein Reduce Midgut Infectivity in Aedes aegypti
TL;DR: It is suggested that the deleted portion of the E2 CRBD represents an important determinant of MRE16 virus midgut infectivity in A. aegypti.
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O'nyong-nyong fever: an epidemic virus diesease in east africa. vii. virus isolations from man and serological studies up to july 1961.
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The distribution and development of eastern equine encephalitis virus in its enzootic mosquito vector, Culiseta melanura.
TL;DR: FA staining of hindguts was determined to be a rapid and reliable method for detection of EEE virus dissemination and replication in Cs.