Journal ArticleDOI
Infection patterns of o'nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae.
Aaron C. Brault,Brian D. Foy,Kevin M. Myles,Cindy L. H. Kelly,Stephen Higgs,Scott C. Weaver,Ken E. Olson,Barry R. Miller,Ann M. Powers,Ann M. Powers +9 more
TLDR
A series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes, that will be a valuable asset in parasite–mosquito interaction and interference research and to serve as tools for antimalaria studies.Abstract:
Arthropod-borne alphaviruses transmitted by mosquitoes almost exclusively use culicines; however, the alphavirus o'nyong-nyong (ONNV) has the unusual characteristic of being transmitted primarily by anopheline mosquitoes This unusual attribute makes ONNV a valuable tool in the characterization of mosquito determinants of infection as well as a useful expression system in Anopheles species We developed a series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes The backbone genome is a full-length infectious cDNA clone of ONNV from which wild-type virus can be rescued Additional constructs are variants of the primary clone and contain the complete genome plus a duplicated subgenomic promoter element with a multiple cloning site for insertion of heterologous genes We inserted a green fluorescent protein (GFP) gene downstream of this promoter and used it to characterize infection and dissemination patterns of ONNV within An gambiae mosquitoes These experiments allowed us to identify atypical sites of initial infection and dissemination patterns in this mosquito species not frequently observed in comparable culicine infections The utility of these ONNVs for studies in anopheline mosquitoes includes the potential for identification of vector infection determinants and to serve as tools for antimalaria studies Viruses that can express a heterologous gene in a vector and rapidly and efficiently infect numerous tissues in An gambiae mosquitoes will be a valuable asset in parasite-mosquito interaction and interference researchread more
Citations
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Journal ArticleDOI
Determinants of Chikungunya and O’nyong-Nyong Virus Specificity for Infection of Aedes and Anopheles Mosquito Vectors
TL;DR: In this article , the authors highlight the complexity and multifactorial aspect of vectorial specificity of the two alphaviruses, and evaluate the level of risk of vector shift by ONNV or CHIKV.
Book ChapterDOI
Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System.
TL;DR: This chapter describes a method for electroporating viral RNA reporters into both fibroblastic and myeloid cells that encode firefly or Renilla luciferase, whose reaction with specific substrates and light emitting activity is a measure of viral RNA translation efficiency.
Posted ContentDOI
Decoding rRNA sequences for improved metagenomics of sylvatic mosquito species
Cassandra Koh,Lionel Frangeul,Hervé Blanc,Carine Ngoagouni,Sébastien Boyer,Philippe Dussart,N Grau,Romain Girod,Jean-Bernard Duchemin,Maria-Carla Saleh +9 more
TL;DR: This expansion of the rRNA reference library improves mosquito RNA-seq metagenomics by permitting the optimization of species-specific rRNA depletion protocols for a broader species range and streamlined species identification by rRNA barcoding.
Journal ArticleDOI
Ribosomal RNA (rRNA) sequences from 33 globally distributed mosquito species for improved metagenomics and species identification
Cassandra Koh,Lionel Frangeul,Hervé Blanc,Carine Ngoagouni,Sébastien Boyer,Philippe Dussart,N Grau,Romain Girod,Jean-Bernard Duchemin,Maria-Carla Saleh +9 more
TL;DR: In this paper , the authors describe a strategy to assemble novel rRNA sequences from mosquito specimens and produce an unprecedented dataset of 234 full-length 28S and 18S rRNA sequence of 33 medically important species from countries with known histories of mosquito-borne virus circulation (Cambodia, Central African Republic, Madagascar, and French Guiana).
Journal ArticleDOI
Identification of mosquito proteins that differentially interact with alphavirus nonstructural protein 3, a determinant of vector specificity
Nathaniel Byers,Paul Burns,Olga Stuchlik,Matthew S. Reed,Jeremy P. Ledermann,Jan Pohl,Ann M. Powers +6 more
TL;DR: In this article , a cell culture model system of the anopheline restriction of CHIKV was developed along with a protein expression strategy to better understand the cellular components controlling alphavirus vector specificity.
References
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Journal ArticleDOI
Re-emergence of Chikungunya and O'nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships.
TL;DR: Phylogenetic trees corroborated historical evidence that CHIK virus originated in Africa and subsequently was introduced into Asia and revealed that ONN virus is indeed distinct from CHIK viruses, and these viruses probably diverged thousands of years ago.
Journal ArticleDOI
Stable germline transformation of the malaria mosquito Anopheles stephensi
Flaminia Catteruccia,Tony Nolan,Thanasis G. Loukeris,Claudia Blass,Charalambos Savakis,Fotis C. Kafatos,Andrea Crisanti +6 more
TL;DR: A transposon, based on the Minos element and bearing exogenous DNA, can integrate efficiently and stably into the germ line of the human malaria vector Anopheles stephensi, through a transposase-mediated process.
Journal ArticleDOI
Germline transformation of the malaria vector, Anopheles gambiae, with the piggyBac transposable element.
Genelle L. Grossman,Cristina S. Rafferty,John R. Clayton,T. K. Stevens,O. Mukabayire,Mark Q. Benedict +5 more
TL;DR: An improvement in the injection technique at the end of the studies resulted in increased G0 hatching, transient expression and EGFP‐expression rates among G1 progeny, and genetic cross data suggest that the insertion site of the element either resulted in, or is tightly linked to, a recessive lethal.
Journal ArticleDOI
Variation in susceptibility to oral infection with dengue viruses among geographic strains of Aedes aegypti.
TL;DR: The comparative susceptibility of 13 geographic strains of Aedes aegypti to oral infection with dengue viruses was studied by feeding the mosquitoes on a virus-erythrocyte-sugar suspension, suggesting that the factors controlling susceptibility were the same for all types.