Journal ArticleDOI
Infection patterns of o'nyong nyong virus in the malaria-transmitting mosquito, Anopheles gambiae.
Aaron C. Brault,Brian D. Foy,Kevin M. Myles,Cindy L. H. Kelly,Stephen Higgs,Scott C. Weaver,Ken E. Olson,Barry R. Miller,Ann M. Powers,Ann M. Powers +9 more
TLDR
A series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes, that will be a valuable asset in parasite–mosquito interaction and interference research and to serve as tools for antimalaria studies.Abstract:
Arthropod-borne alphaviruses transmitted by mosquitoes almost exclusively use culicines; however, the alphavirus o'nyong-nyong (ONNV) has the unusual characteristic of being transmitted primarily by anopheline mosquitoes This unusual attribute makes ONNV a valuable tool in the characterization of mosquito determinants of infection as well as a useful expression system in Anopheles species We developed a series of recombinant alphaviruses, based upon the genome of ONNV, designed for the expression of heterologous genes The backbone genome is a full-length infectious cDNA clone of ONNV from which wild-type virus can be rescued Additional constructs are variants of the primary clone and contain the complete genome plus a duplicated subgenomic promoter element with a multiple cloning site for insertion of heterologous genes We inserted a green fluorescent protein (GFP) gene downstream of this promoter and used it to characterize infection and dissemination patterns of ONNV within An gambiae mosquitoes These experiments allowed us to identify atypical sites of initial infection and dissemination patterns in this mosquito species not frequently observed in comparable culicine infections The utility of these ONNVs for studies in anopheline mosquitoes includes the potential for identification of vector infection determinants and to serve as tools for antimalaria studies Viruses that can express a heterologous gene in a vector and rapidly and efficiently infect numerous tissues in An gambiae mosquitoes will be a valuable asset in parasite-mosquito interaction and interference researchread more
Citations
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Journal ArticleDOI
Pan-viral specificity of IFN-induced genes reveals new roles for cGAS in innate immunity
John W. Schoggins,Donna A. MacDuff,Naoko Imanaka,Maria D. Gainey,Bimmi Shrestha,Jennifer L. Eitson,Katrina B. Mar,R. Blake Richardson,Alexander V. Ratushny,Vladimir Litvak,Rea Dabelic,Balaji Manicassamy,John D. Aitchison,Alan Aderem,Richard M. Elliott,Adolfo García-Sastre,Vincent R. Racaniello,Eric J. Snijder,Wayne M. Yokoyama,Michael S. Diamond,Herbert W. Virgin,Charles M. Rice +21 more
TL;DR: Comparative analysis reveals that the screened ISGs target positive-sense single-stranded RNA viruses more effectively than negative-sensesingle-strander RNA viruses.
Journal ArticleDOI
Tissue Barriers to Arbovirus Infection in Mosquitoes.
TL;DR: The nature of the tissue barriers that arboviruses are confronted with in a mosquito vector and how they might surmount these barriers are explained.
Journal ArticleDOI
Aedes aegypti uses RNA interference in defense against Sindbis virus infection
Corey L. Campbell,Kimberly M. Keene,Douglas E Brackney,Ken E. Olson,Carol D. Blair,Jeffrey Wilusz,Brian D. Foy +6 more
TL;DR: It is shown that silencing RNAi components in Ae.
Journal ArticleDOI
Alphavirus-derived small RNAs modulate pathogenesis in disease vector mosquitoes
TL;DR: It is suggested that an exogenous siRNA pathway is essential to the survival of mosquitoes infected with alphaviruses and, thus, the maintenance of these viruses in nature.
Journal ArticleDOI
Infectious clones of Chikungunya virus (La Réunion isolate) for vector competence studies.
Konstantin A. Tsetsarkin,Stephen Higgs,Charles E. McGee,Xavier de Lamballerie,Rémi N. Charrel,Dana L. Vanlandingham +5 more
TL;DR: Comparison of the growth kinetics and infection rates of the viral isolate CHIKV strain LR2006 OPY1 (CHIKV-LR) and a full-length infectious clone indicate that the infectious clone has retained the viral phenotypes of the original isolate.
References
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Journal ArticleDOI
Promoter for Sindbis virus RNA-dependent subgenomic RNA transcription.
TL;DR: Defective interfering RNAs of Sindbis virus were used and deletion analysis showed that the minimal region required to detect transcription of a subgenomic RNA from the negative-strand template of a DI RNA was 18 or 19 nucleotide upstream and 5 nucleotides downstream from the start of the subgenomics RNA.
Book ChapterDOI
Eastern Equine Encephalomyelitis Virus: Epidemiology and Evolution of Mosquito Transmission
Thomas W. Scott,Scott C. Weaver +1 more
TL;DR: This chapter focuses on the role of mosquitoes in the enzootic transmission of eastern equine encephalomyelitis (EEE) virus and discusses the historic and epidemiologic knowledge, structure and replication, and transmission cycles of the virus.
Journal ArticleDOI
Complexities in the genetic structure of Anopheles gambiae populations in west Africa as revealed by microsatellite DNA analysis
Gregory C. Lanzaro,Yeya T. Touré,John Carnahan,Liangbiao Zheng,Guimogo Dolo,Sekou F. Traore,Vincenzo Petrarca,Kenneth D. Vernick,Charles E. Taylor +8 more
TL;DR: The majority of observed genetic divergence between chromosomal forms of Anopheles gambiae can be explained by forces that need not involve reproductive isolation, although reproductive isolation is not ruled out.
Journal ArticleDOI
Emergence of epidemic O'nyong-nyong fever in Uganda after a 35-year absence: genetic characterization of the virus.
Robert S. Lanciotti,Michelle L. Ludwig,Elly B. Rwaguma,Julius J. Lutwama,Tim M. Kram,N Karabatsos,Bruce C. Cropp,Barry R. Miller +7 more
TL;DR: The data indicate that the recent ONN virus isolate is closely related to the previously published ONN strain isolated in 1959, and phylogenetic analysis of the sequence data reveals that Igbo Ora virus, previously thought to be a separate virus closelyrelated to ONN and Chikungunya, clearly is a strain of ONN.
Journal ArticleDOI
Geographic Genetic Variation in Populations of the Dengue Virus Vector Aedes aegypti
TL;DR: Analysis of FST values, differentiation indexes, and geographic distances separating populations revealed that genetic differences between populations depended on the species' history of migration and colonization.