Journal ArticleDOI
Inside-Out Red Cell Membrane Vesicles: Preparation and Purification
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Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength by centrifugration to equilibrium in dextran density gradients.Abstract:
Plasma membranes purified from human red cells were Converted into small vesicleS by disruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this inversion. The inside-out vesicles were separatcd from right-side-out vesicles by centrifugration to equilibrium in dextran density gradients.read more
Citations
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Journal ArticleDOI
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Journal ArticleDOI
THE ORGANIZATION OF PROTEINS IN THE HUMAN RED BLOOD CELL MEMBRANE A Review
TL;DR: This article will focus on the localization and modes of association of individual major polypeptides within the human red cell membrane.
Book ChapterDOI
Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranes.
Journal ArticleDOI
Preparation and properties of plasma membrane and endoplasmic reticulum fragments from isolated rat fat cells.
TL;DR: The plasma membrane fraction showed a 12–16-fold enrichment of 5′-nucleotidase over the homogenate, and Na + -K + -stimulated ATPase was recovered in highest specific activity in this fraction.
Journal ArticleDOI
Synaptic plasma membranes from rat brain synaptosomes: Isolation and partial characterization
Carl W. Cotman,Dee Ann Matthews +1 more
TL;DR: A fraction enriched in synaptic plasma membranes (SPM fraction) can be prepared on a simple discontinuous gradient in relatively good yield and contains β-N- acetylglucosaminidase, which is not completely washed out of the SPM fraction by salt treatment but is released by low concentrations of Triton X-100.
References
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Journal ArticleDOI
The thiobarbituric acid assay of sialic acids.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
Journal ArticleDOI
Chemiosmotic coupling in oxidative and photosynthetic phosphorylation
TL;DR: The end result of the coupling between the flows through the o/r and h/d pathways in oxidative phosphorylation in mitochondria is that, for the equivalent of each pair of electrons traversing the respiratory chain, up to 3 anhydro-bond equivalents may normally traverse the h/D pathway from adenosine diphosphate plus inorganic phosphate (ADP +Pi) to water.
Journal ArticleDOI
Fine structure in frozen-etched yeast cells
H. Moor,K. Mühlethaler +1 more
TL;DR: The freeze-etching technique, which is a special kind of freeze-drying, allows electron microscopic investigation of cells and tissues in the frozen state of yeast cells and insight into artifacts introduced by common chemical fixation and embedding techniques is obtained.