Insights into the control of RAD51 nucleoprotein filament dynamics from single-molecule studies.
Florian Morati,Mauro Modesti +1 more
TLDR
In this article, the authors describe recent advances in RAD51 control mechanisms obtained specifically using fluorescence-based single-molecule techniques, and describe how modulators control filament dynamics is at the heart of current research efforts.About:
This article is published in Current Opinion in Genetics & Development.The article was published on 2021-09-24 and is currently open access. It has received 3 citations till now. The article focuses on the topics: DNA replication & RAD51.read more
Citations
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Journal ArticleDOI
Human RAD51 Protein Forms Amyloid-like Aggregates In Vitro
Daniel V. Kachkin,Kirill V. Volkov,Julia V. Sopova,Alexander Bobylev,Sergei Fedotov,Sergey G. Inge-Vechtomov,Oxana V. Galzitskaya,Yury O. Chernoff,Aleksandr A. Rubel,Anna Y. Aksenova +9 more
TL;DR: It is demonstrated that a key protein that maintains genome stability, RAD51, has amyloid properties in vitro and in the C-DAG system and the possible biological relevance of this observation is discussed.
Journal ArticleDOI
The Inability to Disassemble Rad51 Nucleoprotein Filaments Leads to Aberrant Mitosis and Cell Death
TL;DR: In this article , the authors demonstrate that without the regulation of these filaments by Srs2 and Rad54, which are known to remove Rad51 from singlestranded and double-stranded DNA, respectively, the filaments strongly inhibit damage-associated DNA synthesis during DNA repair.
References
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Journal ArticleDOI
Rad54: the Swiss Army knife of homologous recombination?
TL;DR: A wealth of genetic, cytological, biochemical and structural data suggests that Rad54 is a core factor of HR, possibly acting at multiple stages during HR in concert with the central homologous pairing protein Rad51.
Journal ArticleDOI
RPA and RAD51: fork reversal, fork protection, and genome stability
Kamakoti P. Bhat,David Cortez +1 more
TL;DR: Current knowledge on the multiple mechanisms by which RPA and RAD51 contribute to genome stability during DNA replication, in particular for replication fork reversal and fork protection are discussed.
Journal ArticleDOI
Rad51 paralogues Rad55-Rad57 balance the antirecombinase Srs2 in Rad51 filament formation.
Jie Liu,Ludovic Renault,Xavier Veaute,Francis Fabre,Henning Stahlberg,Henning Stahlberg,Wolf Dietrich Heyer +6 more
TL;DR: It is proposed that the Rad51 presynaptic filament is a meta-stable reversible intermediate, whose assembly and disassembly is governed by the balance between Rad55–Rad57 and Srs2, providing a key regulatory mechanism controlling the initiation of homologous recombination.
Book ChapterDOI
RecQ Helicases: Conserved Guardians of Genomic Integrity.
Nicolai B. Larsen,Ian D. Hickson +1 more
TL;DR: BLM has been shown to play a critical role in homologous recombination at multiple steps, including end-resection, displacement loop formation, branch migration and double Holliday junction dissolution, and recent evidence has revealed a role for BLM/Sgs1 in the stabilisation and repair of replication forks damaged during a perturbed S-phase.
Journal ArticleDOI
Substrate-Selective Repair and Restart of Replication Forks by DNA Translocases
Rémy Bétous,Frank B. Couch,Aaron C. Mason,Brandt F. Eichman,Maria Manosas,Maria Manosas,David Cortez +6 more
TL;DR: This work shows that SMARCAL1 branch migration and DNA-annealing activities are directed by the single-stranded DNA-binding protein RPA to selectively regress stalled replication forks caused by blockage to the leading-strand polymerase and to restore normal replication forks with a lagging-Strand gap.