Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.
TLDR
The results indicate that IL-10 has important regulatory effects on immunological and inflammatory responses because of its capacity to downregulate class II MHC expression and to inhibit the production of proinflammatory cytokines by monocytes.Abstract:
In the present study we demonstrate that human monocytes activated by lipopolysaccharides (LPS) were able to produce high levels of interleukin 10 (IL-10), previously designated cytokine synthesis inhibitory factor (CSIF), in a dose dependent fashion. IL-10 was detectable 7 h after activation of the monocytes and maximal levels of IL-10 production were observed after 24-48 h. These kinetics indicated that the production of IL-10 by human monocytes was relatively late as compared to the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, tumor necrosis factor alpha (TNF alpha), and granulocyte colony-stimulating factor (G-CSF), which were all secreted at high levels 4-8 h after activation. The production of IL-10 by LPS activated monocytes was, similar to that of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, granulocyte-macrophage colony-stimulating factor (GM-CSF), and G-CSF, inhibited by IL-4. Furthermore we demonstrate here that IL-10, added to monocytes, activated by interferon gamma (IFN-gamma), LPS, or combinations of LPS and IFN-gamma at the onset of the cultures, strongly inhibited the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, GM-CSF, and G-CSF at the transcriptional level. Viral-IL-10, which has similar biological activities on human cells, also inhibited the production of TNF alpha and GM-CSF by monocytes following LPS activation. Activation of monocytes by LPS in the presence of neutralizing anti-IL-10 monoclonal antibodies resulted in the production of higher amounts of cytokines relative to LPS treatment alone, indicating that endogenously produced IL-10 inhibited the production of IL-1 alpha, IL-1 beta, IL-6, IL-8, TNF alpha, GM-CSF, and G-CSF. In addition, IL-10 had autoregulatory effects since it strongly inhibited IL-10 mRNA synthesis in LPS activated monocytes. Furthermore, endogenously produced IL-10 was found to be responsible for the reduction in class II major histocompatibility complex (MHC) expression following activation of monocytes with LPS. Taken together our results indicate that IL-10 has important regulatory effects on immunological and inflammatory responses because of its capacity to downregulate class II MHC expression and to inhibit the production of proinflammatory cytokines by monocytes.read more
Citations
More filters
Journal ArticleDOI
Systemic lupus erythematosus candidate genes in the Italian population: evidence for a significant association with interleukin-10
Sandra D'Alfonso,Marco Rampi,Daniela Bocchio,Gualtiero I. Colombo,Raffaella Scorza-Smeraldi,Patricia Momigliano-Richiardi +5 more
TL;DR: Of the 7 candidate genes tested, only IL-10 was significantly associated with SLE in Italian patients, and this genetic marker represents the only genetic susceptibility factor for SLE found so far in the Italian population.
Journal ArticleDOI
Differential microRNA expression in blood in multiple sclerosis
TL;DR: It is found that RRMS patients in remission had altered expression of miRNAs, and the miR-145 was 3-fold up-regulated in MS patients; its possible use as a diagnostic biomarker in PBMCs, plasma and serum was confirmed by ROC-curve analysis.
Journal ArticleDOI
A Leishmania protein that modulates interleukin (IL)-12, IL-10 and tumor necrosis factor-alpha production and expression of B7-1 in human monocyte-derived antigen-presenting cells.
Probst Peter,Yasir A. W. Skeiky,Yasir A. W. Skeiky,Michelle Steeves,Ana L. Gervassi,Kenneth H. Grabstein,Steven G. Reed,Steven G. Reed +7 more
TL;DR: This is the first report describing IL‐12 production, up‐regulation of co‐stimulatory and intercellular adhesion molecules by monocytic antigen‐presenting cells in response to a protein from a pathogenic microorganism.
Journal ArticleDOI
Differential Regulation of the Stability of Cytokine mRNAs in Lipopolysaccharide-activated Blood Monocytes in Response to Interleukin-10
TL;DR: Investigation of the influence of the cytokine synthesis inhibitory factor interleukin (IL)-10 on the turnover of granulocyte-colony stimulating factor (G-CSF), granulocytes macrophage-colonies stimulating factor, and IL-10 m RNAs in human blood monocytes stimulated with lipopolysaccharide finds that all three mRNAs are destabilized in response to IL- 10 but at different times.
Journal ArticleDOI
Interleukin-10 is a proliferation factor but not a differentiation factor for human myeloma cells.
Zhao Yang Lu,Xue-Guang Zhang,Carmen Rodríguez,John Wijdenes,Zong-Jiang Gu,Brigitte Morel-Fournier,Jean Luc Harousseau,Régis Bataille,Jean-François Rossi,Bernard Klein +9 more
TL;DR: Data show that IL-10 is an IL-6-unrelated growth factor for malignant plasmablastic cells and could be involved in the late phase of MM in vivo.
References
More filters
Book
Molecular Cloning: A Laboratory Manual
TL;DR: Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 years as mentioned in this paper and has been so popular, or so influential, that no other manual has been more widely used and influential.
Journal ArticleDOI
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: A technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, and these "oligolabeled" DNA fragments serve as efficient probes in filter hybridization experiments.
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity
TL;DR: In this article, a technique for conveniently radiolabeling DNA restriction endonuclease fragments to high specific activity is described, where DNA fragments are purified from agarose gels directly by ethanol precipitation and are then denatured and labeled with the large fragment of DNA polymerase I, using random oligonucleotides as primers.
Journal ArticleDOI
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
TL;DR: In this article, the rat pancreas RNA was used as a source for the purification of alpha-amylase messenger ribonucleic acid (RBA) using 2-mercaptoethanol.
Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.