Journal ArticleDOI
Investigation of enrofloxacin residues in broiler tissues using ELISA and LC-MS/MS
Pedro Panzenhagen,Waldemir Silva de Aguiar,Raquel Gouvêa,Andréa M G de Oliveira,Fabiano Barreto,Virginia Léo de Almeida Pereira,Maria Helena Cosendey de Aquino +6 more
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TLDR
The data confirm the efficiency of the ELISA test, and suggest its use as a screening method for enrofloxacin residues in poultry tissues due to its quick results, low price and ease of applicability.Abstract:
This study investigated the efficiency of an enrofloxacin ELISA test kit to detect the presence of enrofloxacin residues in broiler tissues compared with LC-MS/MS. Broiler tissues from 72 samples consisting of 60 breast muscle, six pools of livers (500 g each) and six pools of kidneys (500 g each) were obtained from six different slaughterhouses. Breast muscle from 10 carcasses and pools of livers and kidneys from approximately 200 carcasses of the same flock were collected from each slaughterhouse. ELISA and HPLC were used to identify and quantify the contamination of the samples with enrofloxacin. A total of 72% of the analysed samples contained enrofloxacin residues detected by the ELISA and 22.2% were detected by LC-MS/MS. The mean values of enrofloxacin contamination found in chicken breast by ELISA and HPLC were 8.63 and 12.25 μg kg(-1), respectively. None of the samples exceeded the maximum limit of 100 μg kg(-1) by both methods set by the European Union as well as the Brazilian Agriculture Ministry. All positive samples for enrofloxacin residues detected by LC-MS/MS were also positive by ELISA. These data confirm the efficiency of the ELISA test, and suggest its use as a screening method for enrofloxacin residues in poultry tissues due to its quick results, low price and ease of applicability.read more
Citations
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Two fluorescence quenching immunochromatographic assays based on carbon dots and quantum dots as donor probes for the determination of enrofloxacin
TL;DR: Two fluorescence quenching lateral flow immunochromatographic assays were developed based on carbon dots (CDs) and quantum dots (QDs) as donor signal probes.
Journal ArticleDOI
Determination of veterinary drug residues in food of animal origin: Sample preparation methods and analytical techniques
TL;DR: The three types of methods, microbial, immunological, and physicochemical methods, for screening or determination were described, including microbial inhibition, enzyme-linked immunosorbent assay, colloidal gold immunoassay, fluorescence polarization immunoASSay, biosensor technology, and liquid chromatography-mass spectrometry.
Journal ArticleDOI
Clinical Efficacy and Residue Depletion of 10% Enrofloxacin Enteric-Coated Granules in Pigs.
Zhixin Lei,Qianying Liu,Bing Yang,Jincheng Xiong,Kun Li,Saeed Ahmed,Liping Hong,Pin Chen,Qigai He,Jiyue Cao +9 more
TL;DR: 10% enrofloxacin enteric-coated granules had significant potential for treating APP and MS, and it provided an alternative enro FLXacin palatability formulation.
Journal ArticleDOI
Development of a new dual electrochemical immunosensor for a rapid and sensitive detection of enrofloxacin in meat samples.
Chloé Aymard,Chloé Aymard,Hussein Kanso,Hussein Kanso,María Jesús Serrano,Rafael Pagán,Thierry Noguer,Thierry Noguer,Georges Istamboulie,Georges Istamboulie +9 more
TL;DR: In this paper, a dual electrochemical immunosensor was fabricated for the rapid and sensitive detection of enrofloxacin (EF) antibiotic in meat, which allowed detection of EF at concentrations ranging from 0.005µg to 0.01µgs.
Journal ArticleDOI
Immunochromatographic assays for ultrasensitive and high specific determination of enrofloxacin in milk, eggs, honey, and chicken
TL;DR: In this article , a monoclonal antibody against enrofloxacin, 1H12, was prepared based on the hapten ENR-1, and showed excellent sensitivity with a 50% inhibitory concentration (IC50) of 0.03 ng/mL.
References
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Thomas P. Van Boeckel,Charles H. Brower,Marius Gilbert,Bryan Bt Grenfell,Bryan Bt Grenfell,Simon A. Levin,Timothy P. Robinson,Aude Teillant,Ramanan Laxminarayan,Ramanan Laxminarayan,Ramanan Laxminarayan +10 more
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Interferences in Immunoassay
Jill Tate,Greg Ward +1 more
TL;DR: A wide range of analytes measured by immunoassay including hormones, tumour markers, drugs, cardiac troponin and microbial serology may be affected and laboratories should put processes in place to detect, test and report suspected interferences.
Journal Article
Veterinary Epidemiology, 3rd ed.
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