Isolation of rare cells from cell mixtures by dielectrophoresis
TLDR
The application of dielectrophoretic field‐flow fractionation to the isolation of circulating tumor cells from clinical blood specimens was studied and it is shown that the factor limiting isolation efficiency is cell–cell dielectric interactions.Abstract:
The application of dielectrophoretic field-flow fractionation (depFFF) to the isolation of circulating tumor cells (CTCs) from clinical blood specimens was studied using simulated cell mixtures of three different cultured tumor cell types with peripheral blood. The depFFF method can not only exploit intrinsic tumor cell properties so that labeling is unnecessary but can also deliver unmodified, viable tumor cells for culture and/or all types of molecular analysis. We investigated tumor cell recovery efficiency as a function of cell loading for a 25 mm wide x 300 mm long depFFF chamber. More than 90% of tumor cells were recovered for small samples but a larger chamber will be required if similarly high recovery efficiencies are to be realized for 10 mL blood specimens used CTC analysis in clinics. We show that the factor limiting isolation efficiency is cell-cell dielectric interactions and that isolation protocols should be completed within approximately 15 min in order to avoid changes in cell dielectric properties associated with ion leakage.read more
Citations
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Journal ArticleDOI
Circulating tumor cells: approaches to isolation and characterization
TL;DR: Improvements in technologies to yield purer CTC populations amenable to better cellular and molecular characterization will enable a broad range of clinical applications, including early detection of disease and the discovery of biomarkers to predict treatment responses and disease progression.
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Highly efficient capture of circulating tumor cells by using nanostructured silicon substrates with integrated chaotic micromixers.
Shutao Wang,Kan Liu,Jian Liu,Jian Liu,Zeta Tak For Yu,Xiaowen Xu,Libo Zhao,Thomas H. Lee,Eun Kyung Lee,Jean Reiss,Yi-Kuen Lee,Leland W.K. Chung,Jiaoti Huang,Matthew Rettig,David Seligson,Kumaran N. Duraiswamy,Clifton K.-F. Shen,Hsian-Rong Tseng +17 more
TL;DR: In this paper, a variety of enrichment mechanisms such as immunomagnetic separation based on capture agent-labeled magnetic beads, microfluidics-based technologies that enhance cell-surface contacts, and microfilter devices that isolate CTCs based on size difference are proposed.
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Blood-Based Analyses of Cancer: Circulating Tumor Cells and Circulating Tumor DNA
TL;DR: Recent advances in technologies to analyze circulating tumor cells and circulating tumor DNA are setting the stage for real-time, noninvasive monitoring of cancer and providing novel insights into cancer evolution, invasion, and metastasis.
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Molecular analysis of circulating tumour cells—biology and biomarkers
TL;DR: How circulating tumour cells (CTCs), captured from a minimally invasive blood test—and readily amenable to serial sampling—have the potential to inform intratumour heterogeneity and tumour evolution is described, although it remains to be determined how useful this will be in the clinic.
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Circulating Tumor Cells and Circulating Tumor DNA
TL;DR: These methods allow the detection and characterization of early metastatic spread and will provide unique insights into the biology of metastatic progression of human tumors, including the effects of therapeutic interventions.
References
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Journal ArticleDOI
Isolation of rare circulating tumour cells in cancer patients by microchip technology.
Sunitha Nagrath,Lecia V. Sequist,Shyamala Maheswaran,Daphne W. Bell,Daphne W. Bell,Daniel Irimia,Lindsey Ulkus,Matthew R. Smith,Eunice L. Kwak,Subba R. Digumarthy,Alona Muzikansky,Paula D. Ryan,Ulysses J. Balis,Ulysses J. Balis,Ronald G. Tompkins,Daniel A. Haber,Mehmet Toner +16 more
TL;DR: The CTC-chip successfully identified CTCs in the peripheral blood of patients with metastatic lung, prostate, pancreatic, breast and colon cancer in 115 of 116 samples, with a range of 5–1,281CTCs per ml and approximately 50% purity.
Book
Electromechanics of Particles
TL;DR: In this paper, a detailed account of the electromechanical interactions that govern the behaviour of small particles when an electric or magnetic field is present is presented, with numerous real-world examples.
Journal ArticleDOI
Detection of Circulating Tumor Cells in Peripheral Blood of Patients with Metastatic Breast Cancer: A Validation Study of the CellSearch System
Sabine Riethdorf,Herbert A. Fritsche,Volkmar Müller,Thomas Rau,Christian Schindlbeck,Brigitte Rack,Wolfgang Janni,Cornelia Coith,Katrin Beck,Fritz Jänicke,S. Jackson,Terrie G. Gornet,Massimo Cristofanilli,Klaus Pantel +13 more
TL;DR: The CellSearch system enables the reliable detection of CTCs in blood and is suitable for the routine assessment of metastatic breast cancer patients in the clinical laboratory.
Journal ArticleDOI
Circulating Tumor Cells at Each Follow-up Time Point during Therapy of Metastatic Breast Cancer Patients Predict Progression-Free and Overall Survival
Daniel F. Hayes,Massimo Cristofanilli,G. Thomas Budd,Matthew J. Ellis,A. Stopeck,M. Craig Miller,Jeri Matera,W. Jeffrey Allard,Gerald V. Doyle,Leon W.W.M. Terstappen +9 more
TL;DR: In this paper, the authors reported that ≥5 circulating tumor cells (CTC) in 7.5 mL blood at baseline and at first follow-up in 177 patients with metastatic breast cancer (MBC) were associated with poor clinical outcome.
Journal ArticleDOI
Circulating tumor cells (CTC) detection: clinical impact and future directions.
TL;DR: Cytopathological examination of CTC/CTM, sensitively enriched from blood, represents a potentially useful alternative and can now be employed in routine analyses as a specific diagnostic assay, and be tested in large, blind, multicenter clinical trials.
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